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«РОССИЙСКАЯ АКАДЕМИЯ НАУК ИНСТИТУТ ПРОБЛЕМ ПЕРЕДАЧИ ИНФОРМАЦИИ ИМ. А.А. ХАРКЕВИЧА на правах рукописи ...»

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Приложение 1.

Множественное выравнивание для белков FruR. Участки, соответствующие последовательности HTH, показаны белым шрифтом на темном фоне. Условные обозначения: “*” – абсолютно консервативная позиция;

“:” – позиция с высокой степенью консервативности;

“.” – позиция с низкой степенью консервативности. Обозначения геномов: см. Табл. 2.

FruR(PMU) MKLKLVELAKLAGVSRTTASYVVNGKAKAYRVSDKTIEKVEKLIKQYDFKPNAMAAGLRAGKSRTIGLIVPDFENA FruR(AAC) -----------------------------------------------------MAASLRVGKSKTIGLVIPDFENI FruR(YPK) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVDKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(YPS) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVDKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(YEN) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVDKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(SMA) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVDKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(PCA) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVDKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(PCH) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVDKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(STY) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(STM) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(ECO) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(PLU) --MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENT FruR(VFI) --MTLDEIAKLAGVSKTTASYVINGKAQKYRISEKTQLKVMAVVDEHNYRPDHAASALRAGSSRSFGLIIPDLENT FruR(VCH) --MTLDEIAKLAGVSKTTASYVINGKAQKYRISEKTQHKVMAVVEQYNFRPDHAASALRAGNSRSFGLIIPDLENT FruR(VPA) --MTLDEIAKLAGVSKTTASYVINGKAQKYRISEKTQKKVMAVVDEYNYKPDHAASSLRAGNSRSFGLIIPDLENT FruR(VVU) --MTLDEIAKLAGVSKTTASYVINGKAKKYRISEKTQMKVMAVVEEYNFRPDHAASSLRAGNSRSFGLIIPDLENT FruR(PPR) --MTLDEIAKLAGVSRTTASYVINGKASKYRISEKTQVKVMAVVNEHNFRPDHAATSLRAGSSRSFGLIIPDLENS FruR(PST) --MKLSDIARLAGVSVTTASYVINGKAEQQRISPATVERVKAVVEQYDFRPNPQAAGLRSRHSKTLGFILPDLENP FruR(PFO) --MKLSDIARLAGVSVTTASYVINGKAEQQRISTATVERVRAVVDLHGFTPNPQAAGLRSRHTRTLGFILPDLENP FruR(PPU) --MKLSDIARLAGVSVTTASYVINGKAEQQRISNSTVERVRAVVEAHGFTPNPQAAGLRSRHTRTLGFILPDLENP FruR(PAE) --MKLSDIARLAGVSVTTASYVINGKAAQRRISAATVERVLAVVEEHGYQPDQQAAGLRRGQTRTLGFILPDLENP *:.** ::::*::: **:** FruR(PMU) SFARIATHLEAGFRQKGYQLVIACSNDNPETEMQCAKHLIQRQIDALIVSTTLPADTDFYQRHAN--IPIICFDRY FruR(AAC) SYAKIANNLENRLREKGYQLLIACSNDKVENEQDCVKHLLQRQVDALLVSSALPPNTDFYQ-NAS--VPIIGFDRR FruR(YPK) SYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCVEHLLQRQVDAIIVSTALPPEHPFYQRWAKDPFPIIALDRA FruR(YPS) SYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCVEHLLQRQVDAIIVSTALPPEHPFYQRWAKDPFPIIALDRA FruR(YEN) SYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTALPPEHPFYQRWANDPLPIIALDRA FruR(SMA) SYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTALPPEHPFYQRWANDPLPIIALDRA FruR(PCA) SYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTALPPEHPFYQRWINGGLPIIALDRA FruR(PCH) SYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTALPPEHPFYQRWANDPLPVIALDRA FruR(STY) SYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRA FruR(STM) SYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRA FruR(ECO) SYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRA FruR(PLU) SYTRIANYLERQARQRGYQLLIACSEDQPENEMRCVEHLLQRQVDAIIVSTALPPEHPFYQRWASRSLPIIALDRA FruR(VFI) SYAKLAKLLEFNSRKAGYQILIGCSDDDPETEMNVAHALISRRIDALFVASCLPNGSDYYLGIQEKGTPIIAIDRS FruR(VCH) SYARLAKLLEQNSRQAGYQILIACSDDDPQIEMAAAEALVSRRIDALFVASGIPSASEYYLKLQQSGTPVIAIDRA FruR(VPA) SYARLAKLIEQNSRKAGYQILIGCSDDDPETEKKVAEALISRRIDALFVASGMPSANEYYLKLQNSGTPVIALDRP FruR(VVU) SYARLAKLLEQNSRKAGYQILIACSDDDPETERKVADALVSRRIDALFVATSMADANEFYLAMQKSGTPVIALDRP FruR(PPR) SYAKLAKLLERNARKAGYQLIISCSDDDPDTEMKVAETLLSRRIDALLVASVLPADNDFYPRIQTGGVPVIALDRA FruR(PST) SYARIAKLLEQGARSLGYQLLIASSDDDPVSELQLLQLFRARRCDALLVASCLPPSDASYRDLQLKGMPIIAIDRE FruR(PFO) SYARIAKLLEQGARARGYQLLIASSDDAPDSERQLLQLFRARRCDALIVASCLPAGDDSYRQLQTKGLPIIAIDRV FruR(PPU) SYARIAKQLEQGARARGYQLLIASSDDQPDSERQLQQLFRARRCDALFVASCLPPEDDSYRELQDKGLPVIAIDRR FruR(PAE) SYARLAKQLEQGARARGYQLLIACSDDEPETERQVVNLFRARRCDALIVASCLPAGDDTHARLVAEGLPVVAIDRQ *::::*. :* * ***::*..*:* *. : *: **::*:: :. : *:: : ** FruR(PMU) LDLNK-IRVLTDDEGDSYRLATHLGR--HCEGKRMLFLGALPKLGLSRKRELGFRQALSD-KVNQVDY-LYASQFH FruR(AAC) ISSPKVVNALTDDEGDAYRLTKELLKIPH-Q-KRILFFGALPDLPTSREREEGFRSALRY-RKASAKY-LYGDMFR FruR(YPK) LDREHFISVVGADQEDAQMLAQELRSFPA---ESVLYLGALPELSVSFLREMGFREAWKD-DPRKVEY-LYANSYE FruR(YPS) LDREHFISVVGADQEDAQMLAQELRSFPA---ESVLYLGALPELSVSFLREMGFREAWKD-DPRKVEY-LYANSYE FruR(YEN) LDREHFISVVGADQEDAQMLAQELRSFPA---ESVLYLGALPELSVSFLREMGFREAWKD-DPRKVDY-LYANSYE FruR(SMA) LDREHFISVVGADEEDAFALAQELRTFPA---ESVLYLGALPELSVSFLREQGFRQAWQE-DPRHVDY-LYANSYE FruR(PCA) LDREHFTSVVGADLEDAEMLAQELRKMPA---KSVLYLGALPELSVSFLREQGFRQAWAG-DPNY-LYSNSYE FruR(PCH) LDREHFTSVVGADMEDAEMLAQELRKVPA---ESVMYLGALPELSVSFLREQGFRRAWAE-DPRQVNY-IYANSYE FruR(STY) LDREHFTSVVGADQDDAEMLAEELRKFPA---ETVLYLGALPELSVSFLREQGFRTAWKD-DPNF-LYANSYE FruR(STM) LDREHFTSVVGADQDDAEMLAEELRKFPA---ETVLYLGALPELSVSFLREQGFRTAWKD-DPNF-LYANSYE FruR(ECO) LDREHFTSVVGADQDDAEMLAEELRKFPA---ETVLYLGALPELSVSFLREQGFRTAWKD-DPHF-LYANSYE FruR(PLU) LENEHFISVVGDDFEDAKMLAQEFRQFPA---ESVLYLGALPELSVSFLREQGFREAWKD-DPRKVMY-LYSNSYE FruR(VFI) LDDEHFGCVISEDFDAAYELTKSVVNENV---KSIGLIGALPELNISRERQLGFEAKAKE-K-GLASIMGYGEHFN FruR(VCH) LDDEYFSCVISEDFGAAFELTRSVLTQDV---HSVGLVGALPELNVSREREQGFAMAVKQ-R-GLPTTLGYGEHFN FruR(VPA) MDDEHFCCVISEDFDAAFELTESVLSPEI---KTIGLIGALQDLQVSKERELGFRSACQK-D-DTAMTVGYGLHFS FruR(VVU) LDDEFFSCVVSEDFGAAFELTSSLMSEEI---QTVGLIGALPELNISRERHQGYLSAVKKMG-KQAQS-GYGEHFY FruR(PPR) LDDEIFASVISEDLEGAFELTQTLLNKGV---NSIGLVGAVPELGVSKEREQGFLTAIRRHQPDINMHIAYGDHFS FruR(PST) MDPNFFCSVVSDDHDASLQLTRSLLQTQP---RHIALIGARPELSISRARAAGFEHALDGFA-GTTIV-EHGEAFS FruR(PFO) MDAEHFCSVVSDDREASLHLTESLLDPQP---KQIVLLGARPELSISQERAAGFKQALAGFK-GEVLI-EHAESFS FruR(PPU) LDPAHFCSVISDDRDASRQLAASLLSSAP---RSIALIGARPELSVSQARAGGFDEALQGYT-GEVRR-YQGEAFS FruR(PAE) LDPQRFASVISDDQDAARRLTETLLQPAP---RHIALLGARADLIISHDRAAGFRQALKDFR-GEVIV-EHAEVFS :..: * : *:.. :.**.* * * *:.:

FruR(PMU) KLASAKAFHHWLEK-HEMPEAIFATSLTLLHGVFQILIKRY-HRIPRSLIIATFGHHDMLDLLENKVICCVQNHEK FruR(AAC) KNEAAQCFAQWLEN-NPLPTAIFTTSFTLLEGVLLVLLQKL-GQVPKDLVVATFGHVEMLELLENRVLCSVQDYDK FruR(YPK) REAAGVLFAEWLKT-HPMPQALFTTSFQLLQGVMDVTLKQS-GRLPCNLAIATFGDNELLDFLECPVLAVAQRHRD FruR(YPS) REAAGVLFAEWLKT-HPMPQALFTTSFQLLQGVMDVTLKQS-GRLPSNLAIATFGDNELLDFLECPVLAVAQRHRD FruR(YEN) REAAGALFAQWLKT-HPMPQALFTTSFQLLQGVMDVTLKQS-GRLPSNLAIATFGDNELLDFLECPVLAVAQRHRD FruR(SMA) REAAGALFAEWLKT-HPMPQALFTTSFSLLQGVMDVTLKQR-GRLPTDLAIATFGDHELLDFLECPVLAVAQRHRD FruR(PCA) RVASAAAFMDYLQN-NPMPEALFTTSFPLLQGVMDVNLKIN-GRLPNNLAIATFGDNELLDYLECPVLSVAQRHRE FruR(PCH) RSAAAAVFEEYLKT-HPMPQALFTTSFPLLQGVMDVTLRQS-GRLPNNLAIATFGDNELLDFLECQVLAVAQRHRE FruR(STY) REAAAQLFEKWLET-HPMPQALFTTSFALLQGVMDVTLRRD-GKLPSDLAIATFGDHELLDFLQCPVLAVAQRHRD FruR(STM) REAAAQLFEKWLET-HPMPQALFTTSFALLQGVMDVTLRRD-GKLPSDLAIATFGDHELLDFLQCPVLAVAQRHRD FruR(ECO) REAAAQLFEKWLET-HPMPQALFTTSFALLQGVMDVTLRRD-GKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRD FruR(PLU) RDAAAEVFTKWLET-HPMPQALFTTSFALLQGVMDVTLKHS-GRLPNQLVIATFGDHELLDFLECPVLSVAQRHRE FruR(VFI) SESGKAIFEQWIKN-DTIPDAIITTSYILLEGILDVLIEKP-ELMTR-IKLGTFGDNRLLDFLPIKVNSLPQQFEL FruR(VCH) REEGRKVFAKWVAN-DQLPDAVVATSYTLLEGILDVLLEQP-ELMQK-VRLATFGDNRLLDFLPIRVNSLPQQFEL FruR(VPA) REEGKKVLDKWVQA-DSLPDAIVTTSYTLLEGVLDVMLEKP-ELMSQ-VRLATFGDNRLLDFLPVKINSLPQQFEL FruR(VVU) REEGYRIMHEWLLA-DTIPDAIVTTSYTLLEGVLDVLLEQP-ELVNK-VKLATFGDNRLLDFLPFKVNSLPQQFEL FruR(PPR) QEQGQKQVQQWIDS-EHFPDAILATSYTLFEGILDCLLANP-ELMSK-IHLATFGDNRLLDFLPIKIQSLPQQFEL FruR(PST) RQCGQRLMSEMLQRQGHFPDALITTSYVLLQGVFDVLQSQSLNTTS--LHLGTFGDTQLLDFLPLPVNAMAQQHQL FruR(PFO) RECGKQLMEELLQRLGHLPDALVTTSYVLLQGVFDALHDFPLKSRP--LRLGTFGDTQLLDFLPLPVNAMAQQHQL FruR(PPU) RECGQRLMQQLIDDLGGLPDALVTTSYVLLQGVFDTLQARPVDSRQ--LQLGTFGDNQLLDFLPLPVNAMAQQHGQ FruR(PAE) RECGRRLMEQLLERLGYLPDALVTTAYVLLEGVFDVFQARA-DGWPEGLRVATFGDTQLLDFVPLKVNSIYQQHAL...: :* *:.:*: *:.*:: : :.***. :*: : :. *.

FruR(PMU) IAQTLLHLVLSRM--KSKNVKVSDEVIIREIIE------SRI-- FruR(AAC) VSQSLLTLTFDAMSKKHKN-SVNTTPLTRSIIK------YHC-- FruR(YPK) VAERVLELVLASLDEPHKP-KPGLTRIRRNLFRRGTLSLSRR-- FruR(YPS) VAERVLELVLASLDEPHKP-KPGLTRIRRNLFRRGTLSLSRR-- FruR(YEN) VAERVLELVLASLDEPHKP-KPGLTRIRRNLFRRG--SLSR--- FruR(SMA) VAERVLELVLASLDEPRKP-KPGLTRIRRNLFRRG--SLSR--- FruR(PCA) VAERVLELVLASLDEPKRP-KPGLNRIRRNLYRRG--SLSRM-- FruR(PCH) VAERVLELVLASLDEPRKP-KPGLYRIRRNLYRRG--FLSRL-- FruR(STY) VAERVLEIVLASLDEPRKP-KPGLTRIRRNLYRRG--ILSRS-- FruR(STM) VAERVLEIVLASLDEPRKP-KPGLTRIRRNLYRRG--ILSRS-- FruR(ECO) VAERVLEIVLASLDEPRKP-KPGLTRIKRNLYRRG--VLSRS-- FruR(PLU) IAERVLELVLASLDEKQKP-GPGITRIHRDLCRRG--SLSRKI- FruR(VFI) IADSALALGLNA--SAKRY-QAGIELIPRKIVI----------- FruR(VCH) IADSALALALNA--SAKRY-QTGIELIPRQLKV-------RT-- FruR(VPA) IADSAMELALNA--SAKRY-KPGVELIPRKIVK-------RQ-- FruR(VVU) IADSALELALNA--SAKRY-KPGVELIPRKIVR-------RF-- FruR(PPR) IADNALELALNA--AAGRY-RTGVEVVPRKIKR-------R--- FruR(PST) IADKALQLALAAI-EKDDY-QPGVHAIARAFKQ-------RIHGV FruR(PFO) IADKALELALAAV-EQSDY-QPGVQAIARTFKQ-------RIHRD FruR(PPU) IAATALELALAAI-EEKRY-EPGVHAVGRTFKQ-------RISVA FruR(PAE) IAANALELALRAV-EENDY-RPGLHAVPRQLKL-------R-D- :: :::. :*:

Приложение 2.

Последовательности потенциальных сайтов связывания белка FruR. Указано положение сайта относительно старта трансляции гена. Позиции сайта, совпадающие с консенсусом, показаны прописными буквами, несовпадающие – строчными.

Сайт Геном Оперон Положение Вес Последовательность ctTGAAaCGtTTCAGC -101 4, E. coli fruBKA GCTGAATCGtTTCAat -34 5, agTtAAcCGATTCAGt -124 4, mtlADR aaTtAATCGtTaCAGg -246 3, ptsHI-crr GCTGAATCGATTttat -220 4, tCTGAATCGATTCgat -83 3, manXYZ GCTGAAaCGATaaAGt -133 4, glk GCTGgcgCGATTCAcC -89 4, fbp GCTGAAgCGtTTCAGt -136 5, epd-pgk-fbaA aCTGAAaCGtTTttGC -112 4, edd-eda GtTGAAcCGATTaAGC -160 4, tpiA atTGAcaCGATTCcGC -88 3, gapA agaaAAcCGtTTCAcC 54 3, gpmA GgTGAATCGATaCttt -287 3, pckA GCTtgAaCGATTCAcC -101 4, ppsA cCTGAATCaATTCAGC -122 4, pfkA ctTGAATgGtTTCAGC -222 4, pykF agTGAATCGgTTCAat -252 4, pdhR-aceEF-lpdA GCTGAATCGcTTaAcC -247 4, icdA GCTGAAagGtgTCAGC -263 4, adhE cgTtAAgCGATTCAGC -251 4, aceBAK aCgGAcaCGATTCAaC -282 3, nuoABCDEFGHIJKLMN GCTGAATCGtTaagGt -48 3, nirBDC-cysG GCTGgAagGtTTaAcC -55 4, copA GCTGAAgCGAgaCAcC -221 4, crp GCTGAAgCctTTtAtg -224 3, S. typhi fruBKA ctTGAAaCGtTTCAGC -102 4, GCTGAATCGtTTCAat -36 5, agTtgAaCGATTCAGt -123 4, mtlADR aaTtAATCGtTaCAGg -244 3, ptsHI-crr GCTGAATCGATTttat -218 4, tCTGAATCGATTCgct -82 3, manXYZ GCTGgcgCGATTCAcC -88 4, fbp GCTGAAgCGtTTCAGt -136 5, epd-pgk-fbaA cCTGAAaCGATTttGC -114 4, edd-eda GtTGAAcCGATTaAGC -164 4, tpiA atTGAcaCGATTCcGC -86 3, gapA agaGAAcCGtTTCAcC 52 4, gpmA Сайт Геном Оперон Положение Вес Последовательность GgTGAATCGATattGt -286 4, S. typhi pckA GCTtgAaCGATTCAcC -104 4, ppsA cCTGAATCaATTCAGC -121 4, pfkA ctTGAATgGtTTCAGC -220 4, pykF GtTGAATCGgTTCAGa -254 4, pdhR-aceEF-lpdA GCTGAATCGcTTaAcg -245 4, icdA GCTGAAagGtgTCAGC -266 4, adhE cCTaAAgCGtTTCAGC -251 4, aceBAK aCgGAcaCGATTCAaC -282 3, nuoABCDEFGHIJKLMN GCTGAATCGtTaagGt -46 3, nirBDC-cysG GtTtAAatGcTTCtGC -245 3, copA GCTGAAgCGAgaCAcC -224 4, crp GCTGAAgCctTTtAtg -221 3, S. typhimurium fruBKA ctTGAAaCGtTTCAGC -100 4, GCTGAATCGtTTCAat -34 5, agTtgAaCGATTCAGt -125 4, mtlADR aaTtAATCGtTaCAGg -246 3, ptsHI-crr GCTGAATCGATTttat -220 4, tCTGAATCGATTCgct -84 3, manXYZ GCTGgcgCGATTCAcC -88 4, fbp GCTGAAgCGtTTCAGt -136 5, epd-pgk-fbaA cCTGAAaCGATTttGC -112 4, edd-eda GtTGAAcCGATTaAGC -162 4, tpiA atTGAcaCGATTCcGC -86 3, gapA agaGAAcCGtTTCAcC 54 4, gpmA GgTGAATCGATattGt -288 4, pckA GCTtgAaCGATTCAcC -104 4, ppsA cCTGAATCaATTCAGC -123 4, pfkA ctTGAATgGtTTCAGC -220 4, pykF GtTGAATCGgTTCAGa -254 4, pdhR-aceEF-lpdA GCTGAATCGcTTaAcg -247 4, icdA GCTGAAagGtgTCAGC -266 4, adhE cCTaAAgCGtTTCAGC -251 4, aceBAK aCgGAcaCGATTCAaC -282 3, nuoABCDEFGHIJKLMN GCTGAATCGtTaagGt -48 3, nirBDC-cysG GtTtAAatGcTTCtGC -243 3, copA GCTGgAagGtTTaAcC -85 4, GCTGAAgCGAgaCAcC -226 4, crp ttTGAAaCGtTTCAGC -103 4, P. carotovorum fruBKA GCTGAAaCGATTCAaC -36 5, GCTGAAgCGtTTCAGt -118 5, epd-pgk-fbaA GCTGAATCGAgTaAag -389 4, edd atTGAcaCGATTCcGC -94 3, gapA GCTGAAaCGgTaaAGC -149 4, gpmA GgTtgATCGATTCAcC -118 4, ppsA Сайт Геном Оперон Положение Вес Последовательность cCTGAATCaATTCAGC -135 4, P. carotovorum pfkA atTGAATgGtTTCAGC -240 4, pykF atTtAAaCGgTTCAGC -253 4, pdhR-aceEF-lpdA GCTGAATCGtTTaAct -175 4, icdA GCTGAATgGtgTCAGC -339 4, adhE aCTGAcTCGATTtAGC -257 4, nuoABCDEFGHIJKLMN GCTGAAgCGAgaCAaC -297 4, crp ttTGAAaCGtTTCAGC -105 4, P. chrysanthemium fruBKA GCTGAAaCGATTCAag -37 5, cCTGAATCGATTCAta -195 4, manXYZ GCTGAAatGATTCttg 47 3, aCTGAAgCtgTTtAGC -253 3, glk GCTGAAgCGtTTCAGt -154 5, epd-pgk-fbaA GtTGAAcCGATTaAGC -178 4, tpiA atTGAcaCGATTCcGC -88 3, gapA GCTGAAaCGATaaAGC -146 4, gpmA GgTtgATCGATTCAcC -109 4, ppsA cCTGAATCaATTCAGC -117 4, pfkA GgaagAaCGATTCAGC -144 4, pykA GgTtgAaCGgTTCAGC -254 4, pdhR-aceEF-lpdA GCTGAATCGtTTaAGt -174 5, icdA GCTGAATgGtgTCAGC -314 4, adhE GCTGAAgCGAgaCAaC -307 4, crp atTGAAaCGtTTCAGC -101 5, Y. pestis fruBKA GCTGAAaCGATTCAat -35 5, GCTGAATCGATTtAcC -331 5, ptsHI-crr cCTGAATCGATTCAtt -158 4, manXYZ GCTGAAatGATTttag 59 3, GCTGAAaCGtTTttat -126 4, glk GCTGAAgCGtTTCAGt -160 5, epd-pgk-fbaA GtTGAAcCGATTaAGC -165 4, tpiA atTGAcaCGATTCcGC -79 3, gapA GCaGAATCGATTCgGC -194 4, gpmA GgTtgATCGATTCAcC -194 4, ppsA ctTGAATCaATTCAGC -143 4, pfkA GtTGAATgGtTTCAGC -257 4, pykF aCTatAaCGcTTCAGC -201 4, pdhR-aceEF-lpdA GCTGAATCGgTTaAct -125 4, icdA GCTGAATgGtgTCAGC -308 4, adhE aCTGAcTCGATTtAGC -258 4, nuoABCDEFGHIJKLMN GCTGAtaCctTTCAGC -167 4, nirBDC-cysG GCTGgAagGtTTaAcC -182 4, copA GCTGAAgCGATaCAaC -254 4, crp agTGAAggGtTTCtat -155 3, Сайт Геном Оперон Положение Вес Последовательность ctTGAAaCGtTTCAGC -103 4, Y. enterocolitica fruBKA GCTGAAaCGATTCAat -37 5, GCTGAATCGATTtAtC -286 4, ptsHI-crr cCTGAATCGATTCAtt -221 4, manХYZ GCTGAAaCGtTTttat -131 4, glk GCTGAAgCGtTTCAGt -159 5, epd-pgk-fbaA GtTGAAcCGATTaAGC -162 4, tpiA atTGAcaCGATTCcGC -88 3, gapA atTaAATCGATTCgaC -212 3, gpmA GgTtgATCGATTCAcC -163 4, ppsA ctTGAATCaATTCAGC -145 4, pfkA GtTGAATgGtTTCAGC -258 4, pykF aCTatAaCGcTTCAGC -285 4, pdhR-aceEF-lpdA GCaGAgaCGATTCAct -49 4, GCTGAATCGgTTaAct -176 4, icdA GCTGAATgGtgTCAGC -310 4, adhE aCTGAcTCGATTtAGC -257 4, nuoABCDEFGHIJKLMN aCTGAAaCcATTCAGC -168 5, nirBDC-cysG GCTGtAagGtTTaAcC -141 4, copA GCTGAAgCGATaCAaC -255 4, crp agTGAAggGtTTCtat -156 3, atTGAAaCGtTTCAGC -103 5, Y. pseudotuberculosis fruBKA GCTGAAaCGATTCAat -37 5, GCTGAATCGATTtAcC -333 5, ptsHI-crr cCTGAATCGATTCAtt -156 4, manXYZ GCTGAAaCGtTTttat -127 4, glk GCTGAAgCGtTTCAGt -160 5, epd-pgk-fbaA GtTGAAcCGATTaAGC -163 4, tpiA atTGAcaCGATTCcGC -86 3, gapA GCaGAATCGATTCgGC -276 4, gpmA GgTtgATCGATTCAcC -230 4, ppsA ctTGAATCaATTCAGC -145 4, pfkA GtTGAATgGtTTCAGC -258 4, pykF aCTatAaCGcTTCAGC -273 4, pdhR-aceEF-lpdA GCaGAgaCGATTCAct -37 4, GCTGAATCGgTTaAct -174 4, icdA GCTGAATgGtgTCAGC -308 4, adhE aCTGAcTCGATTtAGC -256 4, nuoABCDEFGHIJKLMN GCTGAtaCctTTCAGC -169 4, nirBDC-cysG GCTGgAagGtTTaAcC -182 4, copA GCTGAAgCGATaCAaC -256 4, crp agTGAAggGtTTCtat -157 3, ctTGAAaCGtTTCAGC -99 4, S. marcescens fruBKA GCTGAAaCGATTCAat -33 5, GCTGAATCGATTtAct -254 4, ptsHI-crr GCTGAAaCGtTTtttg -125 4, glk Сайт Геном Оперон Положение Вес Последовательность GCTGAAgCGtTTCAGt -208 5, S. marcescens epd-pgk-fbaA GtTGAAcCGATTaAGC -164 4, tpiA atTGAcaCGATTCcGC -88 3, gapA GCctgAaCGATTCAGC -150 4, gpmA GgTtgATCGATTCAcC -132 4, ppsA ctTGAATCaATTCAGC -141 4, pfkA GtTGAATgGtTTCAGC -237 4, pykF GCTGAAagGtgTCAGC -306 4, adhE aCTGAcaCGATTtAGC -270 4, nuoABCDEFGHIJKLMN aCTGAcTCGcTTCAGC -167 4, nirBDC-cysG GCTGAAgCGAgaCAaC -301 4, crp GtTGAAaCGtTTCAGt -105 5, P. luminescens fruBA aCTGAAaCGATTCtat -39 4, aCTGAAaCGATTCAat -87 5, fruK GCTGAATCGtTTCAtt -140 5, epd-pgk-fbaA atTGAAcCGATTaAGC -177 4, tpiA atTGAcaCGATTCcGC -89 3, gapA agaGAAcCGtTTCAct 54 4, gpmA GgTtgATCGATTCAcC -113 4, ppsA GtTGAATCaATTCAGC -140 4, pfkA GtTGAATgGtTTCAGC -344 4, pykF GCTGAAagGAaTCAGC -163 4, adhE aCTGAcaCGtTTtAGC -253 4, nuoABCDEFGHIJKLMN GCTGAAaCaATaCAag -240 3, crp GCTtgATtGtTTCAGt -126 4, GCTGAATCGATTCAGt -386 5, V. vulnificus fruBKA GCTGAAagGATTCAGC -364 5, GCTGAATCGgTTCAGC -214 5, GCTGAAcCGATTCAGC -259 5, fruR GCTGAATCctTTCAGC -109 5, aCTGAATCGATTCAGC -87 5, GCTGAAcCGATTCAGt -293 5, V. parahaemolyticus fruBKA GCTGAAagGATTCAGC -271 5, GCTGAATCGATTCAGt -113 5, aCTGAATCGATTCAGC -267 5, fruR GCTGAATCctTTCAGC -109 5, aCTGAATCGgTTCAGC -87 5, GCTGAATCGATTCAGt -265 5, V. cholerae fruBKA GCTGAAagGATTCAGC -243 5, GCTGAAcCGATTCAGC -116 5, GCTGAATCGgTTCAGC -238 5, fruR GCTGAATCctTTCAGC -111 5, aCTGAATCGATTCAGC -89 5, GCTGAATCGATTCAGt -211 5, V. fischery fruBKA GCTGAAagGATTCAGC -189 5, GCTGAATCGATTCAGt -80 5, Сайт Геном Оперон Положение Вес Последовательность aCTGAATCGATTCAGC -221 5, V. fischery fruR GCTGAATCctTTCAGC -112 5, aCTGAATCGATTCAGC -90 5, GCTGAAaCGATTCAGt -241 5, P. profundum fruBKA GCTGAAagGtTTCAGC -219 5, GCTGAAaCGATTCAGC -152 5, GCTGAATCGtTTCAGC -204 5, fruR GCTGAAaCctTTCAGC -137 5, aCTGAATCGtTTCAGC -115 5, agTGAATCGATTCAGC -250 5, P. aeruginosa fruB/ptsI-fruKA GCTGAATCGATTCAct -93 5, fruR GaTtAAaCGtTTCAGC -130 4, P. putida fruB/ptsI-fruKA GCTGAAaCGtTTaAtC -170 4, fruR GaTtAAaCGtTTCAGC -163 4, P. fluorescens fruB/ptsI-fruKA GCTGAAaCGtTTaAtC -158 4, fruR aCaGAATCGAgTaAcg -198 3, P. syringae fruB/ptsI-fruKA GaTtAAaCGATTCAGC -167 4, GCTGAATCGtTTaAtC -203 4, fruR cgTtAcTCGATTCtGt -172 3, Приложение 3.

Множественное выравнивание для белков FruB и PtsI из E. coli и химерного белка из Pasteurellales. Выделены последовательности, соответствующие белкам FruB и PtsI E. coli.

Обозначения геномов: см. Табл. 2.

FruB ECO MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFP PFO MLELTVEQISMGQSAVDKPAALQLLASHLVADGLVADGYLSGLQAREAQGSTFLGQGIAIPHGTPETRDQVFTTGVRLMQFP PST MLELTLGQISMAQSAVDKTAALKLLADHLVADGLVAEGYLTGLMNREQQGSTFLGQGIAIPHGTPETRDLVFTTGVRLMQFP PPU MLELANEQIAMGQKAADKAEALRLLADRLVADGLVAEGYLQGLQAREAQGSTFLGQGIAIPHGTPQTRDLVYATGVRLLQFP PAE MLELDTRQIRMGQRAADKAEALRLLGAALVADGLAAPGYAEGLKAREAQGSTYLGQGIAIPHGTPDTRELVFSTGVRLLQFP *::* :*.: * ** *:: :. ** *.* ** *: ** * **:**:********.:**: * ***:::*** FruB ECO EGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIVASDL PFO DGVDWGDGQIVYLAIGIAAKSDEHLRLLQLLTRALGETDLGQALRRASSPEALLKLLQG--APQELALDAQMIGLGVSADDF PST EGVDWGDGQMVYLAIGIAAKSDEHLRLLQLLTRALGEEDLGQALREAKSPEDLLKLLQG--APQELALDAQMISLGVSADDF PPU EGVDWGDGQMVYLAIGIAARSDEHLRLLQLLTRALGETDLAEALRRASSAEALLKLLQG--APQALALDAQLVGLNVPAEDF PAE EGVDWGDGQQVYLAIGIAAKSDEHLQLLQLLTRALGEADLGPALSAAASAEEVLGLLQG--APQELALDAQLVGLGQNAEDL :** *****.*:****** ***** **: **:.*.:.:. * * :.* : ** *.: * ** ::: *. *.*:

FruB ECO LTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMNDDQPIA PFO EELVWRGARLLRQADCVSNGFAGVLQQVEALPLGDGLWWLHSEQTVKRPGLAFVTPDKPMRYLGQPLSGLFCLASLGEAHQA PST EELVWRGARLLRKADCVSNGFAAVLQQVEALSLGDGLWWLHSEQTVKRPGLAFVTPDKPIRYLGQPLTGLFCLASLGEAHQA PPU DELAWRGARLLQRADCVDSGFAAVLQQAEPLPLGEGLWWLHSERQVRQPGLAFITPQQPLRYRDQPLNGLFCLASLGAAHEA PAE DELAWLGARLLKKAGCVENGFAAVLQQTEPLPLGDGLCWLHSEQLVKRPGLAFVTPAQPLQHQGQLVTGLFCLASLGEAHQA *.* *:.*..*. *. : :.* **:*:.*. :..:*.. :.:.: *..:*. * FruB ECO VLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKP PFO LLERLCALLIEGRGHELGRATSSRKVLEVLGGELP-ADWPSARIALANAHGLHARPAKILAQLAKSFDGEIRVRIVDGQDSA PST LLERLCLLLIEGRGHELGHATNSRVVLEALGGEVP-AEWPTQQIQLANAHGLHARPAKILAQLAKEFEGEVRVRVLETGESA PPU LLERLCEVLIEGRGQVLYQATSSRAVLEVLGGEVP-ADWPSARVVLANPHGLHARPAKVLAQLAKGFEGEIRVRLVDSAQPA PAE LLERLCDLLLEGRGAELVRATSSRSVLAALGGELP-PDWPSARAVLANPHGLHARPAQALAQLAKGFAGEIRVRLADSEAAP :*:**. :*::.:. * :* : :..: * : :. : * *******. *.: * *.:: * :.

FruB PtsI ECO ANGRSLMKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA MISG PFO VSVKSLSKLLSLGARRGQVLEIIAEPSIAADALPALLAAIEEGLGEEVEPLPAVSQ--QIADIAEVLSAPA-SGSLLQA PST VSAKSLSKLLSLGVRRGQTLEFIAEPSIAADALPALIAAVEQGLGEEVEPLPAASEPEAAPTPVAVAKPINAPA-AGSVLSA PPU VSVKSLSKLLSLGARRGQALELVAEPSIAADALPVLLAAIEQGLGEEVEPLPQSVA----PIADDVPEVLQAPA-AGSRIQG PAE VSAKSLSKLLALGARRGQTLEFSAEPAIAEDALPALLAAVREGLGEEVEALAEEALP--DAVGEAEEDARPAPLRAGERLQA.. :** *:::**.::*: *.: *: : * :**.: *: ****. :..

PtsI ECO ILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFLSGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELE PFO IPAAPGIAIGPAHIQVQQAIDYPLR---GESAAIERERLKQALADVRQDIQGLIERSKA---KAIREIFITHQEMLDDPELT PST VPASPGIAIGPAHVQVLQTFDYPQK---GESVAAERERLQTAIGEVRRDIENLIQRSKS---KAIREIFITHQEMLDDPELI PPU VGAAPGIASGPAHVCVEREFDYPLR---GESCALERQKLREALATVNGELQALVLRSD----KAIGEIFVTHQEMLADPALT PAE IAASPGIASGPAHVQVAQRFEFQPR---GESPAHERERLLRAKRAVDEEIVGLVERSTV---KAIREIFVTHREMLDDPELA : *:**** * * : : :.:. * :::.. :: : : : : ** * :* * * PtsI ECO QEIIALIKDKHMTADAAAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVAADLTPSET PFO DEVDTRLK-QGESAEAAWMAVIEAAAKQQESLHDALLAERAADLRDIGRRVLAQLCGVQTP--AEPEQPYILVMDEVGPSDV PST EQRLN-DNESAAAAWATVIEAAAVQQEQLKDALLAERAADLRDVGRRVLAQICGVETV--AAPDEPYILVMDEVGPSDV PPU DDVEQRLA-QGESAAAAWMAVIEAAARQQEALHDALLAERAADLRDIGRRVLAQLCGVQAQ--VEPEQPYVLVMTEVGPSDV PAE EQVQLRLN-RGESAEAAWSRVVEDSAAQQEALHDALLAERAADLRDLGRRVLARLCGVEAP--REPEQPYILVMDEVGPSDV :: : :* ** *:* * * *.* * *****:**:*:*:*.: *:: :: :** :: **:.

PtsI ECO AQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYLILDAVNNQVYVNPTNEVIDKMRAVQEQVASEK PFO ARLDPARVAGILTARGGATAHSAIVARALGIPALVGAGAAVLLLKPGTPLLLDGQRGRLHVDADAATLQRASEERDTREQRL PST ARLDPAQVAGILTARGGATAHSAIVARALGIPALVGAGDEVLLLKPGTVLLLDSQRGRLTVAPDQATLQRAVEDRDAREQRL PPU ARLDPNRVAGIVTAQGGATAHSAIVARALGIPAVVGAGASILLLESGTPLLLDGQRGVVSVAPPADELQRALAERDLREQRL PAE ARLDAQRVAGILTARGGATSHSAIIARALGIPALVGAGAAVLGLEPGTALLLDGEHGWLQVAPSTEQLQQAAAERDARQQRQ *:*: :* *::* ** *:*::*:**:* :**:**:* ::. *:**... : *. ::: ::..

PtsI ECO AELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVR PFO KAAAEQRHQPAHTTDGHAVEVFANIGESAGVISAVEQGAEGIGLLRTELIFMAHPQAPDEATQEAEYRRVLDGLAGRPLVVR PST KAAAAARMEPAVTRDGHAVEVFANIGDSTGTPAAVEQGAEGVGLLRTELLFMAHSQAPDEATQEAEYRRVLTDLGGRPLVVR PPU QAAWANRFEPAITRDGHAVEVFANIGDSNGIAKVVEQGAEGVGLLRTELIFMAHPQAPDVATQEAEYRRVLDGLDGRPLVVR PAE ARADAQRLEPARTRDGHAVEVCANLGDTAGAARAVELGAEGVGLLRTEFVFMNNARAPDLATQEAEYRRVLDALDGRPLVAR : ** * *** *** **:*... ****:** ***::**. * * * *: *.:.::.* PtsI ECO TMDIGGDKELPYMNFPKEENPFLGWRAIRIAMDRREILRDQLRAILRASAFGKLRIMFPMIISVEEVRALRKEIEIYKQELR PFO TLDVGGDKPLPYWPIAKEENPFLGVRGIRLTLQRPQIMEAQLRALLRSADNRPLRIMFPMVGSVEEWRQARDMTERLRLEIP PST TLDVGGDKPLPYWPIAKEENPFLGVRGIRLTLQRPDVMESQLRALLRAADSGPLRIMFPMIGTLEEWREARAMTERLRAEIP PPU TLDVGGDKPLPYWPIAAEENPFLGVRGVRLTLQRPQVMEDQLRALLRAADQRPLRIMFPMVGQVHEWREARAMVERLRAEIP PAE TLDVGGDKPLPYWPIPHEENPYLGLRGIRLTLQRPQILETQLRALFRAAGERPLRVMFPMVGSLDEWRQARDLALRLREEIP *:*:**** *** :. ****:** *.:*::::* :::. ****::*:: **:****: :.* * * : *:

PtsI ECO DEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEG PFO ------VPDLQLGIMIEVPSAALLAPVLAKEVDFFSVGTNDLTQYTLAIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHG PST ------VSDLQLGIMIEVPSAALIAPVLADFFSIGTNDLTQYTMAIDRGHPTLSAQADGLHPSVLQLIDMTVRAAHANG PPU ------VADLQLGIMVEVPSAALLAAQLADFFSIGTNDLTQYTLAIDRGHPSLSAQADGLHPAVLSLIDMTVRAAHAHG PAE ------LADLQLGIMVEVPSAALLAPVLADFFSVGTNDLTQYTLAIDRGHPSLSAQADGLHPAVLQLIDMTVRAAHAEG.:::*:*:*.*:** :* **:******:*********:*:***: :* : : *:**.**..: *:**.* PtsI ECO KWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVNKFIEEKTIC PFO KWVGVCGELAADPLAVPVLVGLGVDELSVSGRSIAEVKARIRELSLTQTQTLAQQALAVGSANEVRALVEAL------ PST KWVGVCGELAADPLAVPVLVGLGVDELSVSARSIGEVKACVRELTLSSAQQLAQKALTAGSAAEVRALVEAV------ PPU KWVGVCGELAADPQAVAVLLGLDVDELSVSARSIAEVKALVRQADHQTARALAREALQQDSAAAVRALVERY------ PAE KWVGVCGELAADPLALPLLVGLGVDELSVSARSIALVKAGVRELQLVAARGLARKALGLASAAEVRALVEAEVQ---- **.*:*****.* * :*:*:.:**:*:*. ** :* :*: :: **.:** :: : :**:

Приложение 4.

Множественное выравнивание нуклеотидных последовательностей fruR-fruB/ptsI из Pasteurellales. Условные обозначения: кодинующие последовательности показаны заглавными буквами, некодирующие – строчными;

потенциальные сайты связывания FruR показаны белым на темном фоне;

консервативные позиции помечены звездочками.

Обозначения геномов: см. Табл. 2.

fruR PPU GGCGATATCGCTGAGTTTCAC----------------------------------------cgaattttccttgttattacc PFO CGCGATATCACTGAGTTTCAA--------------------------------------cccgggatttccttgttttttc PST TGCAATATCGCTCAGTTTCACcccgaaaattcctttagttggccaggcggctcaggaccgtcgaattgttttcgccgtggc PAE GGCGATATCACTGAGTTTCAA-------------------------------------aaccgatctctactggaggcgacg ** ***** ********** ** ** ** * PPU ggggctgcctgtaaggccggaccgggtagtttgacccggtacaggcatcacgcgcagacgaccattgtcgcaat-tgt-ccg PFO -gagcttgccgcgacatt---ttcgccaatcctacccgattcaggca---------ggcgaccattgtctcagcgcat-ccg PST ggcgatcattacaccagaccgtaggaaattcctaagtacttcaagtattt------taggaagtctcctacagc-gat-cgg PAE acaagttgggcttcaagg--atcaatcattatcgggtaacgtgccaatttaattaagtgaatcgattcagcaaaagattccg * ** * * * ** *** PPU ac-aggatggggctttttactgggcagattatcgagtaacgtg---gccgtctggt-ca-gattaaacgtttcagctg--cc PFO ac-aagttggacttcaaggatggg-acattatcgagtaacgtg---ccaatcaatc-ta-gattaaacgtttcagcaa--gc PST ac-cgactgggcttcaatga-acgcacagaatcgagtaacgtg---ccgggcaattgta-gattaaacgattcagcac--gc PAE gcgaagccggcgccccctgccccaggctgcctcgcgcagcccgaacgcggcgccgcctacgctatggcaggtcaatgacaac * ** *** * * * * * * ** * *** * PPU gaattttctgc------------ctcggctgccacccgcgcaa-ggttgctgaa-ctggccctttatgtgaatttcacaa-- PFO gtattttctacgttttagacgcctttggccggttctgccgtga-aaccgccaaaactgcctcctgaagggaagctgacaagcg PST ctgttgtctgc-tgcaatcgagatgaaactgatgtccg-atag-gatattcgatgggcatcactttcatcctcttagctgtcg PAE aagcggatcaccgcccctggctgtacggaaggctcccgcgagccgctttccgctaccgccggcacatcccgcgcccgcgcct * * * fruB/ptsI PPU -caatactccagta---cccgaacgggaact--gcaaaaggagaaggtc------------------ATGCTCGAGCTCGCCA PFO cctaagctgcaaccat-tcaaaacaatacct--ggcgtcacacgacgccaaaaaggagatcgca---ATGCTCGAGCTCACTG PST atcacatgtgatcgccattaaaacaagaatcaggtggcgccctggcgctgcacaggagtcaaggcaaATGCTCGAACTCACTC PAE -cggcgcggcgggacgatccggacgtgagacggggagccgttcgctcctgaggaggtttcc------ATGCTCGAACTCGATA ** * * ******** *** Приложение 5.

Множественное выравнивание нуклеотидных последовательностей fruR-fruB межгенных промежутков из Vibrionales. Условные обозначения: см. Приложение 4.

Обозначения геномов: см. Табл. 2.

fruR VPA ATCCAGTGTCAT---------------------tat--tctcaaccct--a---ttct--gtgcgcgcg-atcata----tt VVU ATCCAGTGTCAT---------------------tat--tacctaccct--a---atct--gtgcgcctg-atcataga--tt VFI ATCTAATGTCAT---------------------tggg-taaggatcctcaa---tttt--gtg-gttcggatcatagaaata VCH ATCCAGTGTCAT---------------------taa---gggggtctc--g---ttttatgtgcgtctg-atcatagaa-cg PPR ATCTAACGTCATgatgagttcttatcgtttgcttataatagagattaaaaagagattt--gtgcgcttg-atcatataatcg *** * ***** * * * *** * * ****** VPA --aatacatcaacgatc--ttac-g-ata-agttg-aattataagctgaaccgattcagtataaaagctgaaaggattcagc VVU cgaat---tcca-gatc--tt-c-gcgtc-tgttg-aattatacgctgaatcgattcagtataaaagctgaaaggattcagc VFI tagat---ccaatcagc--tta--g-gt---gttg-aattatacgctgaatcgattcagtataaaagctgaaaggattcagc VCH c-gat---ttt-tgatc--cta--gcctagtgttg-aattatacgctgaatcgattcagtattaaagctgaaaggattcagc PPR caaat--------gatcctttatagaaca--attgcaatcata-gctgaaacgattcagtatgtctgctgaaaggtttcagc ** ** * * *** *** *** ****** *********** ********* ****** VPA aaaa-t-ctaaaaattgaccaaaatcacc-aa---aat----ccggcaa--tg--cagtaagtcttcgt-ctaggct-t-ta VVU aaaa-t-ctgcaaatagacatacatcacctaattgaatgattttgtcgaattgaacagcat-tctcggtattgaact-cgtt VFI aaa--t-ttgaaaa---gcgtg-at-----------at----ttg-cg-------c--tat-tttt--------act-cata VCH aaaagtaccgttgatt--caca-atc----------------tcgtcca------ctacaaaggtc------agattgtgtc PPR aaagct-cta-aaat--gt-taagtggc---------t----tt----------actgaaactttt-------aatt---tc *** * * * * * * * VPA agcaata--ca-gct-caag---gggtgt--ttaacgttag-attttt--aaaacatttt--gctaagtc--tt----ttaa VVU ggccgtgtccacgttgtaaataaaagtgccgtt--tgctga-atttttttaaaccaatt---gctgaatcggttcagcttaa VFI a---atg--cgaatt-taa-------tgc-------gtaga-aat-----aaa----ttt--gttgaact--tt----tt- VCH ga--gtatcca-gcagcaag-----at-c-------gtgagtattc----gag--gtttt--gctgaat---tt----tt- PPR a---gca--aaagctgaaacgattcagc----------------------------ttttaggttgaag----------ta ** ** *** * VPA -------aaaattt--gc--cact-ta-----gctgaatcgattcagtatcgaaagaag-g--tttagcta-a-gacac-- VVU gttaagcaaaagttaagcaacgctgtgaaagtgcttaa-cgctccaa-atctaagggtgtgcttttaacca-aaggcgcttt VFI ------------ttgagt---gcttt------gctgaatcgattcagt--tt------g----ttttgc-a-a-ggca--- VCH ------------tt--gc---cctttg----tgctgaaccgattcagc--tcaaaggaa-------aacgagacgggatgaa PPR ------------tc-aac----ttata-----attaca-cgacgt-g--tcgatgggtatg---ttaactg---ggtatg- * ** * * ** * VPA --aacaaaactag-------agcaa----gact-----------gaatacatttt------ta-----at---ttga-ttgg VVU taggcaaagacagtctgttaagcaaagcagacttcttaaacaaagaagacctctcacaccgtagagaaacaggttgaaccga VFI ---ataaa--ta----------c------gatt-----------gatta--tc---------ag----------tga----a VCH t-gacaagg-tatt-------gcttc---aact-----------gaaaacctgcagcgatacat---cacatctcg--ccta PPR --aatagagattttt------acctc---ggtt------------attagtactaat----cag----acaaataga--aga * * * ** * * fruB VPA tacgaat----acctta-aggctggagaagcaccATGCTTAAGCTAAACAAGAACGACATTACCCTCTCGCAATCAGCGGCA VVU gagaaatgacgacgcgatacgctaagaaggcaacATGCTCAAATTAACTTCATCAGATATTACGCTCCAGCAGAGCGCCGAC VFI catagat----a-gcgagaggct---aagac-ccATGCTGACATTAACAAAGAATGACATTACGCTGCAGCAGTCAGCAGCG VCH tagaggc----agacagga-gtt---aaga----ATGTTAGAACTCACTACACAAGATATTCAATTGCAGCAACACTTTGCG PPR cagaaat------ccagtggagt--gaattca--ATGTTGTCACTATCTCAGAACGATATTCAGTTAAAGCAAACCGCTACC * * * *** * * ** *** * *** Приложение 6.

Множественное выравнивание для белков PurR и RbsR. Условные обозначения: см.

Приложение 1. Обозначения геномов: см. Табл. 2.

PurR(STY) MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH-YSPSAVARSLKVNHTKSIGLLATSSEAAYFAE PurR(STM) MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH-YSPSAVARSLKVNHTKSIGLLATSSEAAYFAE PurR(ECO) MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH-YSPSAVARSLKVNHTKSIGLLATSSEAAYFAE PurR(YPS) MATIKDVAKHAGVSTTTVSHVINKTRFVAENTKAAVWAAIKELH-YSPSAVARSLKVNHTKSIGLLATSSEAPYFAE PurR(YPK) MATIKDVAKHAGVSTTTVSHVINKTRFVAENTKAAVWAAIKELH-YSPSAVARSLKVNHTKSIGLLATSSEAPYFAE PurR(PLU) MATIKDVAKHAGVSTTTVSHVINKTRFVAEDTKAAVWAAIKALN-YSPSAVARSLKVNHTKSIGLLATSSEAPYFAE PurR(PCA) MATIKDVAKRAGVSTTTVSHVINKTRFVAEETKAAVRAAIKELH-YSPSAVARSLKVNHTKSIGLLATSSEAPYFAE PurR(PMU) MATIKDVAKMAGVSTTTVSHVINKTRFVAAETEKLVLQAIQELN-YSPSAVARSLKVNTTKSIGMIVTSSEAPYFAE PurR(HIN) MATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLN-YSPSAVARSLKVNTTKSIGMIVTTSEAPYFAE PurR(HDU) MATIKDVAKLAGVSTTTVSHVINKTRFVAEETSKTVWNAVQKLN-YSPSAVARSLKINTTKSIGMIITTSEAPYFAE PurR(VPA) MATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVMKAVDELN-YAPSAVARSLKCNSTRTIGMLVTQSTNLFFSE PurR(VCH) MATIKDVARLAGVSTTTVSHVINKTRFVAETTQEKVMEAVKQLN-YAPSAVARSLKCNTTRTIGMLVTQSTNLFFSE PurR(VVU) MATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVMKAVDELN-YAPSAVARSLKCNTTRTIGMLVTQSTNLFFSE PurR(VFI) MATIKDVAKLAAVSTTTVSHVINKTRFVAEATQKRVWEAVEELN-YAPSAVARSLKCNTTRTIGMLVTQSFNPFFAE PurR(PPR) MATIKDVARMAGVSTTTVSHVINKTRFVAEATQKKVLAAVDDLN-YAPSAVARSLKCNTTKTIGMLVTKSTNPFFAE RbsR(PPR) MATIKDVAKHVGVSTSTVSHVLNKTRFVSEDISTRVLAAVDELN-YAPSALARSLKVKNTRTFGMLVTTSTNPFFGE RbsR(VFI) MATIKDVAKHASVSTSTVSHVLNKTRFVSEDISERVMAAVAELN-YAPSALARSLKVNHTKTFGMLVTTSTNPFFGE RbsR(VVU) MATMKDIAKLAGVSTSTVSHVINKTRFVSEEIAERVNHAAKELNYFAPSALARSLKVNRTNTIGMLVTTSTNPFFGE RbsR(VPA) MATMKDIAKLAGVSTSTVSHVINKTRFVSEEISERVNNAAKELNYYAPSALARSLKVNRTKTIGMLVTTSTNPFFGE RbsR(VCH) MATMKDIARLAGVSTSTVSHVINKSRFVSDEIAERVNNAAQQLN-YAPSALARSLKMNRTKTIGMLVTTSTNPFFGE RbsR(STY) MATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLN-YAPSALARSLKLNQTRTIGMLITASTNPFYSE RbsR(STM) MATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLN-YAPSALARSLKLNQTRTIGMLITASTNPFYSE RbsR(ECO) MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN-YAPSALARSLKLNQTHTIGMLITASTNPFYSE RbsR(PCA) MATMKDVARLAGVSTSTVSHVINNNRFVSDAIREKIMKAVDDLN-YAPSALARSLKINQTRTIGMLLTASSNPFYAE RbsR(PLU) MATMKDVARFAGVSTSTVSHVINNNRYVSDGVKKKVNDAIEQLN-YAPSALARSLKIKQTKTIGMLVTTSNNPFYAE RbsR(PMU) MATMKDIARIAKVSTSTVSHVINNSRFVSDEIRDKVMHVVKELN-YTPSALARSLKVKETKTIGLLVTATNNPFFTE RbsR(HIN) MATMKDIARLAQVSTSTVSHVINGSRFVSDEIREKVMRIVAELN-YTPSAVARSLKVRETKTIGLLVTATNNPFFAE RbsR(PFO) MATIKDVAALAGISYTTVSHVVNKTRPVSEEVRLKVEAAIKSLD-YVPSAVARSLKAKTTATIGLLVPNSLNPYFAE RbsR(PPU) MATIKDVAALAGISYTTVSHVLNKTRPVSEQVRLKVEAAIIELD-YVPSAVARSLKARSTATIGLLVPNSVNPYFAE RbsR(PST) MATIKDVAALAGISYTTVSHVLNKTRPVSEPVRLKVEAAIAQLD-YVPSAVARSLKAKTTSTIGLLIPNGMNPYFAE RbsR(PAE) MATMKDVAAMAGVSFTTVSHVVNRTRPVSDAVRKKVEDAIAQLH-YVPSAVARSLKVRTTSIIGLLVPNSTNPYFAE ***:**:*. :* :*****:* * *: : *. : ***:*****. * :*::. :: * PurR(STY) IIEAVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKRVDGLLVMCSEYPEPLLSMLEEYRHIPMVVMDWGEAKAD PurR(STM) IIEAVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKRVDGLLVMCSEYPEPLLSMLEEYRHIPMVVMDWGEAKAD PurR(ECO) IIEAVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKAD PurR(YPS) VIEAVENSCYSKGYTLILCNSHNNLDKQKAYLAMLAQKRVDGLLVMCSEYPDQLLGMLEDYRNIPMVVMDWGTARGD PurR(YPK) VIEAVENSCYSKGYTLILCNSHNNLDKQKAYLAMLAQKRVDGLLVMCSEYPDQLLGMLEDYRNIPMVVMDWGTARGD PurR(PLU) IIESVENSCYSKGYTLILCNSHNNIDKQKAYLAMLAQKRVDGLLIMCSEYPEQLLGMLEDYRNIPMVVMDWGQARSD PurR(PCA) IIEAVENSCYAKGYTLVLCNSHNDIGKQRAYLSMLAQKRVDGLLVMCAEYPPELLGMLEDYRSIPMVVMDWGQMHND PurR(PMU) IIHAVEEHCYRQGYSLFLCNTQNNVEKIKNHLEMLIKKRVDGILVMCAEYTQDSLDLLANFTSLPLVVMDWGPD-NK PurR(HIN) IIHSVEEHCYRQGYSLFCVTHKMDPEKVKNHLEMLAKKRVDGLLVMCSEYTQDSLDLLSSFSTIPMVVMDWGP--NA PurR(HDU) IVLAVEEHCYQQGYSLFLCNTQNEPEKIQNHLDMLIKKRVDGILVMCSEYTRNSLELFN-ATNIPMVVMDWGKA-HE PurR(VPA) VIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTEELKEMLDRHSDIPKVVMDWGPE-SS PurR(VCH) VIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTQELQDMLDAHKDIPKVVMDWGPE-TS PurR(VVU) VIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTEELREMLDKHADIPKVIMDWGPI-SS PurR(VFI) VMHGVENYCYKQGYTLFMCNTEGDLEKQKHYLRMLAEKRVDGLLVMCSDLNEQLLTLLEKNTELPMVIMDWGPD-SP PurR(PPR) VIHGVEEYCYNAGYTLILCNTEGNLVKQRDYLRMLAEKRVDGLLVMCSDIDQDLLDLLARKSDLPMVIMDWGPE-SP RbsR(PPR) VVKGVERRCYEYGYNLILCNTEGDIARMHSNLDTLLQKRVDGLLLMCSEVDNEEFNLFARHQPVPTVVMDWGPIDF RbsR(VFI) VVKGVERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLLLMCTEIENQVLELFSRYQPVPTVVMDWGLIDF RbsR(VVU) VVKGVERSCYQKGYNLILCNTEGDHERMKSSIMTLLQKRVDGLILMCSSLEGERLDVFERYPDIPVVVMDWGPMLF RbsR(VPA) VVKGVERSCYHKGYSLILCNTEGDNERMRQSINTLLQKRVDGLILMCSSLEGERIDVFERYPDIPVVVMDWGPMLF RbsR(VCH) VVKGVERSCYHQGYNLILCNTEGDNQRMKASINTLLQKRVDGLLLMCSTLEGERLDVFDRYPDIPIVVMDWGPILF RbsR(STY) LVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMERYPSIPTVMMDWSPFDGE RbsR(STM) LVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMERYPSIPTVMMDWSPFDGE RbsR(ECO) LVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDG RbsR(PCA) VVRGVERCCYERGYSLILCNTEGDRDRMSHSLETLLQKRVDGVLLMCTESHRPLPEMMSRYPSIPMVMMDWAPFEG RbsR(PLU) IVRGVERSCYERGYSLILCNTEGDTKRMDYSMETLLQKRVDGVLIMCTENQHNSCDVLRRYPPLPVVMMDWSPFDI RbsR(PMU) VVSGVEQYCHQHHYNLILSNTEGDTERLRHNLQTLIQKQVDGLLLMCSDARIQIGKDLN--VNLPFVVMDWWPTEE RbsR(HIN) VMAGVEQYCQKNQYNLIIATTGGDAKRLQQNLQTLMHKQVDGLLLMCGDSRFQADIELA--ISLPLVVMDWWFTEL RbsR(PFO) LARGIEDYCERNGYCVILCNSDDNPDKQRSYLRVLLEKRIDGLIVASAGGDSGLAQGLAG-VKTPMVIVDRGLEGVD RbsR(PPU) LARGIEDACERNGYCVILCNSDDNPQKQRSYLRVLLEKRIDGLVVASVGQDDDLLQSLAS-VRTPMVIVDRELEGVD RbsR(PST) LARGIEDYCERNGFCVILCNSDDNPEKQRSYLRVLLEKRVDGLIVSSVAGDTSVTCGLTE-VRTPLVIVDRELQGID RbsR(PAE) LSRGIEDCCERNGYCVILCNSDDNPQKQANYLRVLQEKRIDGLVLASAGGDGALAKGLAN-MRTPTVIVDEGVEGVD :.

:* * : :.. : : :.*::**:::. : * *::* PurR(STY) -FTDTVIDNAFAGGYMAGRYLVERGHRDIGVIPGPLERNTGAGRLAGFMKAMEEALINVPDNWIVQGDFEPESGYHA PurR(STM) -FTDTVIDNAFAGGYMAGRYLVERGHRDIGVIPGPLERNTGAGRLAGFMKAMEEALINVPDNWIVQGDFEPESGYHA PurR(ECO) -FTDAVIDNAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQGDFEPESGYRA PurR(YPS) -FTDSIIDNAFEGGYLAGRYLIERGHRDIGAIPGQLARNTGGGRHQGFLKALEEANIPVREEWIVQGDFEPESGYKA PurR(YPK) -FTDSIIDNAFEGGYLAGRYLIERGHRDIGAIPGQLARNTGGGRHQGFLKALEEANIPVREEWIVQGDFEPESGYKA PurR(PLU) -FTDTIIDNAFHGGYLAGRYLIERGHRDIGAIPGPLARNTGGGRHQGFLKALKEANIPIKEEWVIQGDFEPESGYKA PurR(PCA) -FTDTIIDNAFEGGYMAGRYLIERGHRDIGAIPGIQERNTGSGRYLGFLKALKEADITVRNEWVVQGDFEPESGYKA PurR(PMU) -HTDIIQDHSFEGGYLATKYLIDHGHKDIGIIAGELTKTTAKTRYEGFIKAMNEAGLKINPDWVMEGFFEPEDGYEC PurR(HIN) -NTDVIDDHSFDGGYLATKHLIECGHKKIGIICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYEC PurR(HDU) -KNDRIIDNSFNGAYLATRHLIDNGHKDIGVISGYLTKTICSERLEGFIKAMKEANLPIREEWILEGDFEPESGFEQ PurR(VPA) -RADKIIDNSEEGGYLATKYLIDNGHTDIACLSGHFEKLACQERIAGFRRAMAEAKLPINEDWILEGNFECDTAVLV PurR(VCH) -HADKIIDNSEEGGYLATKYLTDRGHTEIACLSGHFVKAACQERIQGFRRAMAEAKLTVNEDWILEGNFECDTAVLA PurR(VVU) -QADKIIDNSEEGGYLATKYLIDNGHTKIACLSGHFEKAACQERIQGFKRAMKEANITLNNEWILEGNFECDTAVMA PurR(VFI) -RTDKIIDNSEEGGYLATKHLIENGHTHIACITGQADKVTCKERVRGFERAHIDANLTFNPEWILEGDFECASASRA PurR(PPR) -LTDKIQDNAEQGGYVATKHFIDNGHEKIGCLSGHSEKSTCRERLKGFNKAMAEAGITVNNNWIIDGDFECESAVEA RbsR(PPR) -PSDKIQDNSHHGGYLATRHLIAQGHTQIGCLTGPLDKLPAQQRLSGFVQAMEEAGLTINKSWIASGNFECEGGEKA RbsR(VFI) -PSDKIQDNSHHGGYLATKHLIEQGHTEIGCLTGPLNKLQAQQRLSGFVQAMEESGLEINKNWIASGNFECEGGEAA RbsR(VVU) -SSDKIQDNSLRGGYLAANHLIQMGHKEIGCITGPLIKHQAQMRYEGYKRALNEHGLPFNPAWIVEADFECEGGYQA RbsR(VPA) -TSDKIQDNSLRGGYLAAKYLIDCGHTEIGCITGPLIKHQAQMRYEGYKRAMNEAGLEFNANWIIESDFECEGGYQA RbsR(VCH -ASDKIQDNSLQGGYMAAKHLIECGHKEIGCITGPLIRHQAQMRYEGYKRALAEAGIAINPDWIVESDFECEGGYQA RbsR(STY) GDSDLIQDNSLLGGDLATQHLIDKGFTRIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDA RbsR(STM) GDSDLIQDNSLLGGDLATQYLIDKGFTRIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDA RbsR(ECO) -DSDLIQDNSLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDA RbsR(PCA) -AIDVIKDNSLLGGEIATNYLISRGYKKIACIAGPKDKTTAYNRLEGYRQAMQLAGLSVPTDYEIFGDFEFETGYRA RbsR(PLU) -VGDVIQDNSLLGGEMATNHLISCGFKKIACIAGPQDKTPANHRLKGYRKAMANAGLTIPDGYEIYSDFEFAGGFKS RbsR(PMU) -KADKIYENSEYGGYLATKQLIEKGHQHIAIITGNLKKSLAQNRLDGYKKALKEANIPLREDCIIESHFDFEGGKRG RbsR(HIN) -NADKILENSALGGYLATKALIDAGHRKIGIITGNLKKSVAQNRLQGYKNALSEAKIALNPHWIVESHFDFEGGVLG RbsR(PFO) --ADLVRIDHEYGAYLATRHLLELGHRDIATIGGPASTSVAQMRQAGFARALKEAGVEVAPSRMLESDFTSTGGYNA RbsR(PPU) --ADLVRIDHEQGAYLATRHLLELGHRDVAYIGGPAETGVTQLRLSGFRRAMAEAGAPVPGSRVLHCDFTSPGGHAA RbsR(PST) --ADMVRIDHEQGAWLATRHLLDLGHRHIACIAGPRESAVAELRLAGYRRAMQQASIEVGAGWAVHSEFTSPAGHEA RbsR(PAE) --ADRVQIDHEMGAYLATRHLLELGHRDIACIGGPLSTKVSTLRVEGYRRAMAKAGLEVRPQWLLESEFSSPGGYHA *:. *. :*. : *. :. : * * *: *. *.

PurR(STY) MQQILSQS--HRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSVIGYDNVRNARFFTPALTTIHQPKDSLGETAFNM PurR(STM) MQQILSQS--HRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSVIGYDNVRNARYFTPALTTIHQPKDSLGETAFNM PurR(ECO) MQQILSQP--HRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDNVRNARYFTPALTTIHQPKDSLGETAFNM PurR(YPS) MHQILTQK--HRPTAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFSPALTTIHQPKERLGETAFAM PurR(YPK) MHQILTQK--HRPTAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFSPALTTIHQPKERLGETAFAM PurR(PLU) MHQILNQK--QRPTAVFCGGDVMAMGAICAADELGLRVPQDISVIGYDNIRNARYFSPALTTIHQPKERLGQMAFSM PurR(PCA) MHQILAQK--QRPTAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRHARFFTPALTTIHQPKERLGQSAFSM PurR(PMU) MNKILAQD--TLPTAVFCCNDVMALGAISAIGEKGLRVPEDISIIGYDNIHASRFYSPPLTTIHQSKSRLGARAVTL PurR(HIN) MNRLLTQE--KLPTALFCCNDVMALGAISALTEKGLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAINI PurR(HDU) MNNLLRLD--KLPTAIFCFSDTIALGVLSALSEKGIAVPQDISIIGYDNTHSSRFFSPPLTTIHQSKSRLGVKALNL PurR(VPA) ADKITAME--KRPTAVFCFNDTMALGLMSRLQQNGIKVPDDVSVIGYDNIELAEYFSPPLTTIHQPKRRVGKNAFEI PurR(VCH) ADKIIAMD--KRPTAVFCFNDTMALGLMSRLQQKGIRIPEDMSVIGYDNIELAEYFSPPLTTVHQPKRRVGKNAFEI PurR(VVU) ADKIASWQ--DRPTAVFCFNDTMALGLMSRLQQLGLRIPEDISVIGYDNIELAEYFSPPLTTIHQPKRRVGKNAFEI PurR(VFI) VDKILAIEENKRPTALFCFNDIMALAAISKIQQSGLRVPEDISVIGYDNIELSAYFSPPLTTIHQPKRRVGKTAVEI PurR(PPR) ANQYIAMK--DRPTAIFCFNDIMAMALISTFEQAGVRVPDDISVIGYDNIDLAPYFSPPLTTIHQPKRRLGKTAIEI RbsR(PPR) FEELYARG--SLPTALFVCNDMMAMGVINTANKKGISVPNDISIVGYDDIKLAKYITPSLTTIHQPKHRLGQQAVDT RbsR(VFI) FNEIHAKG--KLPTALFVCNDMMAMGMINCASKKGISVPNDISIVGYDDIKMAKYITPSLTTIHQPKHRLGQQAVDS RbsR(VVU) FKKIVAKG--PLPSALFVCNDMMAMGVINAANEVGVEIPHDLSIIGYDDIHIAKFMTPSLTTIHQPKYRLGQAAVET RbsR(VPA) FKKMAERG--TLPSSIFVSNDMMAMGVINAANELGIKVPDDLSIIGYDDIHIAKFMSPSLTTIHQPKYRLGQAAVET RbsR(VCH) FEKLYQRG--KLPSALFVSNDMMAMGVIQAASQRGLRVPDDLSLIGYDDVHIAKFMTPALTTIHQPKYRLGKAAVDT RbsR(STY) MQALLAHQ--QRPQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGELAIDV RbsR(STM) MQALLAHQ--QRPQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGELAIDV RbsR(ECO) MRQLLSHP--LRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDV RbsR(PCA) MQQLLALE--DKPEAVFTSNDAMAVGVYHALYQAGLSIPQDMAVIGYDDIELARYMSPPLTTVHQPKDELGELAVDT RbsR(PLU) MQELLQHP--EPPEAVFAGNDAMAVGAYQALYQAGLSVPDDISIIGYDDIDLAPYMIPPLTTIHQPKDELGKLAVDT RbsR(PMU) MEKVLALQ--PRPTAVFACSDSIAFGAYQTVWHQGLHIPQDISIIGYDNITLAEYMSPPLTTINQPKAELGKLAVET RbsR(HIN) IQSLLTQS--SRPTAVFCCSDTIAVGAYQAIQQQGLRIPQDLSIMGYDDIELARYLSPPLSTICQPKAELGKLAVET RbsR(PFO) ASILL--ER-NPPSAIFAGNDMIGIGVLRAAAERNVRVPAELSVIGFDDIQMSRYVYPALTTVGQSILQLGEMAAEV RbsR(PPU) AAQVL--EG-KRPSAIFAGNDMIGFGVLRAAAERNISVPGELSVIGFDDIELSRYVYPSLTTVGQSIRELGESAASL RbsR(PST) AGRLL--AE-HAPTAIFAGNDVIAIGVLRAAAERSIRVPQDLSVIGFDDIQISRYVYPALTTVGQSIMQLGETAAGM RbsR(PAE) ALGLLGGEG-ERPTAIVAGNDMIGIGVLRAAAELNIRVPHELSVIGFDDIELSRFVFPALTTVGQSIRLLGEIAAQL * ::..* :.... : :* :::::*:*: : : *.*:*: *. :* * PurR(STY) LLDRIV--NKRE---ESQSIEVHPRLVERRSVADGPFRDYRR PurR(STM) LLDRIV--NKRE---ESQSIEVHPRLVERRSVADGPFRDYRR PurR(ECO) LLDRIV--NKRE---EPQSIEVHPRLIERRSVADGPFRDYRR PurR(YPS) LLDRIV--SKRE---DPQTIEVHPKLVERRSVADGPFRDYRR PurR(YPK) LLDRIV--SKRE---DPQTIEVHPKLVERRSVADGPFRDYRR PurR(PLU) LLDRII--NKRE---DAQTIEVHPRLVERRSVVDGPFVDYRR PurR(PCA) LLDRIT--SKRE---DAHVIEVHPTLIERRSVADGPFRDYRR PurR(PMU) LFERIN--EKSE---ERAVIEMHPELVIRKSVKSRL------ PurR(HIN) LLERIT--HKDEGVQQYSRIDITPELIIRKSVKSIL------ PurR(HDU) LLERIKLGHKEY---QPHTIEFHPELVLRHSVRNLNKSEE-- PurR(VPA) LLERIK--DKEH---EKRVFEMQPEIVIRNTVK-KLN----- PurR(VCH) LLERIK--DKEH---ERRIFEMHPEIVERDTVK-DLTKS--- PurR(VVU) LLERIK--DKEH---DKRVFEMHPELVVRDTVK-KIN----- PurR(VFI) LLERIK--DKDH---ERRVFEMQPEVVTRSSVSNRLK----- PurR(PPR) LMERVK--DKNH---ERRVFEMNPELVIRKSVK-DLN----- RbsR(PPR) LLDKIQ--SQRE---TNLIIQLEPTLVERDSVKKM------- RbsR(VFI) LLEKIA--TKSE---TNRVFQLEPTLMVRDSVKTLSN----- RbsR(VVU) LLKKIN--KETA---EVQVVQLEPTLIERNSVVEWHDNEI-- RbsR(VPA) LVRRLD--DKSN---EAQVVQLEPTLVVRNSVTNFS------ RbsR(VCH) LLYRLE--NPDT---TAQVVQLEPTLVVRNSVRKLG------ RbsR(STY) LIHRMA--QPAL---QQQRLQLTPVLTVRGSV---------- RbsR(STM) LIHRMA--QPAL---QQQRLQLTPVLTVRGSV---------- RbsR(ECO) LIHRIT--QPTL---QQQRLQLTPILMERGSA---------- RbsR(PCA) LLYRLE--HPNT---EPNVLVLTPELMVRQSVQ--------- RbsR(PLU) LLYRMD--NPES---DPKLLVLTPTLIERSSVSRS------- RbsR(PMU) LLQRIK--NPTA---KHRTLLLEPQLVWRESVRQLYQEKRG- RbsR(HIN) LLQRIK--NPNE---NYRTLVLEPTCVLRESI---YSLK--- RbsR(PFO) LLRRIATP-DLA----TDQRIVTPSIVLRESTAPLSGVFTEYR RbsR(PPU) LLTRIATPRQGA----AEQRIVAPRIVLRESTGPRPDLFNDYR RbsR(PST) LLSRIASPHATP----VEKRLVKPCVVVRESTAAPNTSSNE- RbsR(PAE) LLQRIAER-EEP----MQRRVVAPALVLRESTAPPLVRV--- *. ::.* *:

Приложение 7.

Последовательности потенциальных сайтов связывания белка RbsR. Указано положение сайта относительно старта трансляции гена. Позиции сайта, совпадающие с консенсусом, показаны прописными буквами, несовпадающие – строчными. 1) Сайты, обнаруженные в геномах, где отсутствует ген регулятора.

Сайт Геном Оперон Положение Вес Последовательность E. coli TCAGCGAAACGTTTCGcTGA -32 6, rbsDACBKR ctAGCGAAACGTTTCGAcGg -110 6, S. typhi rbsDACBKR cCAGCGAAACGTTTCGcTag -31 6, ctAGCGAAACGTTTCGAcGg -112 5, S. typhimurium rbsDACBKR cCAGCGAAACGTTTCGCTAg -33 6, TtAGCGAAACGTTTCGATGg -110 6, P. carotovorum rbsDACBKR TtAGCGAAACGTTTCGCTGg -33 6, TtATCGAAACGTTTCGATAg -111 6, P. luminescens rbsDACBKR atAGCGAAACGTTTCGCTAA -33 5, TCAtCGAAACGTTTCGATGA -45 6, P. multocida rbsDACBKR TtAtCGAAACGTTTCGATaA -38 6, H. influenzae rbsDACBKR TCAtCGAAACGTTTCGATGt -36 6, V. cholerae rbsDACBKR TtAcgcAAACGTTTCGATGA -72 5, V. fischeri rbsDACBKR cCATCGAAACGTTTCGATGA -225 6, V. parahaemolyticus rbsDACBKR gCATCGAAACGTTTCGATGA -95 6, TCATCGAAACGTTTCGATGA -73 6, V. vulnificus rbsDACBKR cCATCGAAACGTTTgcgTAA -119 5, P. profundum rbsDACBKR 1) TtAGCGAAACGTTTCGCTcT -32 6, rbsDK Y. pestis 1) TtAGCGAAACGTTTCGCTcT -32 6, rbsDK Y. pseudotuberculosis Приложение 8.

Последовательности потенциальных сайтов связывания белка PurR. Указано положение сайта относительно старта трансляции гена. Позиции сайта, совпадающие с консенсусом, показаны прописными буквами, несовпадающие – строчными.

Сайт Геном Оперон Положение Вес Последовательность AgGCAAACGTTTaCcT -61 4, E. coli purR gaGCAAACGTTTcCac 27 4, AaGaAAACGTTTtCGc -357 4, prsA ACGCAAACGTgTGCGT -168 4, purC AgGaAAACGaTTGgcT -122 4, purA AaGCAAACGgTgattT -23 3, ACGCAAACGTTTtCGT -54 5, purT ACGCAAACGgTTtCGT -91 4, purL ACGCAAtCGgTTaCcT 185 4, purB ACGCAAcCGTTTtCcT -86 4, purEK ACGCAAACGTTTtCtT -71 4, cvpA-purF tCGCAAACGTTTGCtT -80 4, purMN gCGCAAACGTTTtCGT -122 4, purHD AtGCAAtCGgTTaCGc -68 4, guaBA ACGCAAtCGTTaaCcT -76 3, yhhP ACGaAAACGaTTGCtT -83 4, codBA AaGCAAACGTTTGCGa -233 4, upp-uraA AgGaAAACGTTTcCGc -66 4, pyrC cgGaAAACGTTTGCGT -103 4, pyrD gCGCAAgCGTTTtCca -237 3, carAB AataAAcCGTTTGCGc -91 3, pyrLBI AaGaAAcCGgTTGCGc -133 4, speAB AtGCAAACGaTTtCaa -82 4, glnB AaGagAACGaTTGCGT -105 4, gcvTHP ggtaAAACGaTTGCGc 22 3, folD AgGtAAtCGTTTGCGT -133 4, glyA ACGCAAACGTTcataT -94 3, serA ACGCAAtCGaTTaCGT -153 4, tsx AaGCAAACGTTTGCca -226 4, gltS ACGCAAtCGTTgcCGT -114 4, yieG ACGatAACGTTTGCGc -273 4, yjcD AgctAAACGTTTGCtT -131 3, gtGCgAcCGTTTtCGT 28 3, rnt gCGtAAcCGaTTGCaT -109 3, xseA tgGCAAACGTTTGCtT -69 4, yicE AgGttAACGaTTGCGT -160 3, yhhQ ACGCAAACGaTTtacT -223 4, ydiJ AgGCAAACGTTTaCcT -57 4, S. typhi purR ggGCAAACGTTTcCac 29 4, AaGaAAACGTTTtCGc -472 4, prsA ACGCAAACGTgTGCGT -132 4, purC AgGaAAACGaTTGgcT -121 4, purA AaGCAAACGgTgattT -23 3, Сайт Геном Оперон Положение Вес Последовательность ACGCAAACGTTTtCGT -50 5, S. typhi purT ACGCAAACGgTTtCGT -91 4, purL ACGCAAACGgTTaCcT 183 4, purB ACGCAAcCGTTTtCcT -88 4, purEK ACGCAAACGTTTtCtT -73 4, cvpA-purF tCGCAAACGTTTGCtT -77 4, purMN gCGCAAACGTTTtCGT -124 4, purHD AtGCAAcCGaTTaCGT -63 4, guaBA ACGCAAtCGTTaaCcT -78 3, yhhP ACGaAAACGTTTGCtT -75 4, codBA AaGCAAACGTTTGCGa -351 4, upp-uraA ACGaAAACGTTTcCGc -66 4, pyrC AgGaAAACGaTTGCta -100 4, pyrD gCGCAAgCGTTTtCta -237 3, carAB AaGaAAcCGgTcGCGc -133 3, speAB AaGagAACGTTTGCGT -107 4, gcvTHP ggtaAAACGaTTGCGc 20 3, folD AgGtAAtCGTTTGCGT -133 4, glyA ACGCAAtCGaTTaCGT -155 4, tsx gCGCAAcCGgTTGCGc -273 4, gltS AaGCAAACGaTTGCGa -175 4, AaGCAAACGTTTGCta -186 4, yicE tCGCAAtCGTTTGCtT -67 4, ACGCAAACGTTTcCta 81 4, yieG ACGatAACGTTTGCGc -272 4, yjcD AgctAAACGTTTGCtT -129 3, gtGCgAcCGTTTtCGT 30 3, rnt ACGtAAtCGgTTGCaT -107 4, xseA AgGttAACGaTTGCGT -107 3, yhhQ ACGCAAACGaTTaaGc -101 4, ydiJ AgGCAAACGTTTaCcT -57 4, S.


typhimurium purR ggGCAAACGTTTcCac 29 4, AaGaAAACGTTTtCGc -474 4, prsA ACGCAAACGTgTGCGT -130 4, purC AgGaAAACGaTTGgcT -121 4, purA AaGCAAACGgTgattT -23 3, ACGCAAACGTTTtCGT -38 5, purT ACGCAAACGgTTtCGT -89 4, purL ACGCAAACGgTTaCcT 185 4, purB ACGCAAcCGTTTtCcT -86 4, purEK ACGCAAACGTTTtCtT -71 4, cvpA-purF tCGCAAACGTTTGCtT -15 4, purMN gCGCAAACGTTTtCGT -122 4, purHD AgGCAAcCGaTTaCGT -79 4, guaBA ACGCAAtCGTTaaCcT -76 3, yhhP AaGCAAACGTTTGCcT -77 4, codBA AaGCAAACGTTTGCGa -350 4, upp-uraA ACGaAAACGTTTcCGc -64 4, pyrC Сайт Геном Оперон Положение Вес Последовательность AgGaAAACGaTTGCta -102 4, S. typhimurium pyrD gCGCAAgCGTTTtCta -237 3, carAB AaGaAAcCGgTcGCGc -133 3, speAB AaGagAACGTTTGCGT -107 4, gcvTHP taGCAAACGTTTtCtT -40 4, folD AtGCAAtCGTTTGCGT -125 4, glyA gCGCAAcCGgTTGCGc -272 4, gltS AaGCAAtCGTTTGCGT -191 4, tCGCAAtCGTTTGCtT -69 4, yicE ACGCAAtCGTTgcCGT -117 4, yieG ACGatAACGTTTGCGc -272 4, yjcD AgctAAACGTTTGCtT -129 3, gtGCgAcCGTTTtCGT 30 3, rnt ACGtAAtCGgTTGCaT -109 4, xseA AgGttAACGaTTGCGT -109 3, yhhQ ACGCAAACGaTTaaGc -101 4, ydiJ AgGCAAACGaTTaaca -61 3, Y. pestis purR ACGCAAtCGTTTtCGT -86 4, purT ACGCAAACGgTTtCGT -131 4, purL ACGCAAACGcTTaCcT 194 4, purB ACGCAAcCGTTTtCcT -115 4, purEK ACGCAAACGTTTtCtT -75 4, cvpA-purF tCGCAAACGTTTGCcT -241 4, purMN ACGCAAtCGTTTtCGc -110 4, purHD AgGCAAtCGaTTaCGc -90 4, guaBA ACGCAAACGaTTaaGg -30 3, yhhP AgGCAAACGTTTGCGa -138 4, upp-uraA tCGCAAACGTTTaCtT -253 4, pyrD tCGCAAcCGTTTaCtc -212 3, carAB AgGtAAtCGTTTGCta -323 3, pyrBI AaGgtAtCGTTTGCGT -97 3, gcvTHP ggtaAAACGaTTGCGc 22 3, folD AgcCAAtCGTTTGCGT -324 4, glyA AgGCAAtCGTTTGtaT -9 4, serA AaGCAAtCGTTTGCGc -183 4, gltS gCGCAAACGaTTGCtT -19 4, yicE AgatAAtCGTTTGCcT -205 3, yjcD ACGgAAACGaTTaCGT -72 4, gCGtAAtCGaTTGCcT -107 3, xseA AgGCAAACGaTTaaca -63 3, Y. pseudotuberculosis purR ACGCAAtCGTTTtCGT -84 4, purT ACGCAAACGgTTtCGT -129 4, purL ACGCAAACGcTTaCcT 183 4, purB ACGCAAcCGTTTtCcT -115 4, purEK ACGCAAACGTTTtCtT -73 4, cvpA-purF tCGCAAACGTTTGCcT -281 4, purMN ACGCAAtCGTTTtCGc -179 4, purHD AgGCAAtCGaTTaCGc 8 4, guaBA Сайт Геном Оперон Положение Вес Последовательность ACGCAAACGaTTaaGg -171 3, Y. pseudotuberculosis yhhP AgGCAAACGTTTGCGa -169 4, upp-uraA tCGCAAACGTTTaCtT -255 4, pyrD tCGCAAcCGTTTaCtc -241 3, carAB AgGtAAtCGTTTGCta -323 3, pyrBI AaGgtAtCGTTTGCGT -97 3, gcvTHP ggtaAAACGaTTGCGc 22 3, folD AgcCAAtCGTTTGCGT -322 4, glyA AgGCAAtCGTTTGtaT -111 4, serA AaGCAAtCGTTTGCGc -185 4, gltS gCGCAAACGaTTGCtT -68 4, yicE AgatAAtCGTTTGCcT -205 3, yjcD ACGgAAACGaTTaCGT -72 4, gCGtAAtCGaTTGCcT -109 3, xseA AgGCAAACGaTTaaca -68 3, P. carotovorum purR AaGaAAACGTTTtCGT -353 4, prsA ACGCAAACGTTTtCGT -60 5, purT ACGCAAACGgTTtCGT -112 4, purL ACGCAAACGcTTtCcT 183 4, purB ACGCAAcCGTTTtCcT -131 4, purEK ACGCAAACGTTTtCtT -89 4, cvpA-purF tCGCAAACGaTTGCcT -128 4, purMN ACGCAAACGTTTtCGc -158 4, purHD AgGCAAtCGaTTaCGc -78 4, guaBA gCGCAAACGTTgtCGT -81 4, yhhP AgGCAAtCGTTTGCGa -241 4, upp-uraA cCGCAAACGTTTtacT -125 3, pyrD taGCAAcCGTTTaCtT -238 4, carAB AaGCAAcCGaTTGCGa -288 4, pyrBI AaGatAtCGTTTGCGc -63 3, gcvTHP ggtaAAACGaTTGCGc 20 3, folD AgctAAtCGTTTGCGT -151 3, glyA AaGCAAtCGTTTGtaT -97 4, serA AgGCAAcCGTTTtCGT -130 4, tsx taGCAAACGTTTGCtT -73 4, yicE ACGCAAACGTTTaCtT -85 4, yjcD gCGtAAtCGaTTGCcT -110 3, xseA ACGacAACGTTTGCGc -151 4, yhhQ gCGCAcACGaTTGCGa -238 3, ydiJ AaGaAAACGTTTcCGT -294 4, P. luminescens prsA ACGCAAACGgTTtCGT -90 4, purL ACGCAAACGcTTaCcT 197 4, purB ACGCAAtCGTTTtCcT -85 4, purEK ACGCAAACGTTTtCGT -71 5, cvpA-purF tCGCAAACGTTTGCtT -81 4, purMN ACGCAAACGTTTtCGT -282 5, purHD AgGCAAcCGaTTaCGT -79 4, guaBA AaGCAAACGTTTGCGa -161 4, upp-uraA Сайт Геном Оперон Положение Вес Последовательность ttGCAAACGTTTttcT -98 3, P. luminescens speAB AaGatAtCGTTTGCGT -107 4, gcvTHP ggGaAAACGaTTGCGc 22 4, folD AtGCAAtCGTTTGCGT -125 4, glyA AaGCAAtCGTTTGCGT -191 4, gltS ACGCAAACGaTTGCtT -67 4, yicE AgctAAtCGTTTGCGT -132 3, yjcD ACGtAAtCGgTTGCcT -109 4, xseA AgGtAAACGcTTGCcT -128 3, ydiJ AtGCAAACGaTTGtGa -310 3, P. multocida prsA AtGCAAACGTTTGCtT -84 4, purC ACGCAAACGTTTtCGT -84 5, purL AgGCAAACGaTTaCtT 183 4, purB AgGCAAtCGTTTGCtT -77 4, purEK ACGCAAACGTTTtCtT -68 4, cvpA-purF tCGCAAACGTTTGCtT -73 4, purMN gCGCAAACGTTTGCGT -74 4, purHD-glyA AaGCAAACGTTTGCcT -77 4, codBA ttGCAAACGTTTttcT -98 3, speAB taGCAAACGTTTtCtT -40 4, folD gCGCAAACGaTTaCGc -103 4, rpiA-serA AgcCAAACGaTTGCtT -113 4, yjcD AaGtAAACGTTTGCGT -127 4, deoD AaGaAAtCGTTTataa 59 3, pckA ACGCAAtCGTTTGCta -65 4, glpX taGCAAACGaTTGCGT -166 4, yiiU ttGCAAcCGTTTGCtT -101 4, HD AtaaAAACGaTTGCGa -283 3, H. influenzae prsA taGCAAACGTTTGCtT -31 4, purC AtGCAAACGTTTGCtT -1 4, purL taGCAAACGTTTGCcT -77 4, purEK ACGCAAACGTTTtCtT -84 4, cvpA-purF tCGCAAACGTTTGCtT -62 4, purMN AaGCAAACGTTTGCGT -65 5, purHD taGCAAACGcTTtCtT -44 3, folD ACGagAAaGTTTtCtT -333 3, glyA ACGCAAtCGTTTaCtT -36 4, rpiA ACGCAAACGTTTaCtT -85 4, yjcD AtGCAAACGaTTaCtc -75 4, glpX gaGtAAtCGTTTGCaT -102 3, yiiU ttGCAAACGgTTGCtT -94 4, HD ggGgAAACGaTTGCGa -271 3, H. ducreyi prsA taGCAAtCGTTTGCtT -91 4, purC ACGCAAACGTTTGCtT -40 4, purT AtGCAAtCGTTTGCtT -1 4, purL AgGCAAACGaTTaCtT 185 4, purB AaaCAAtCGTTTGttT -68 3, purE Сайт Геном Оперон Положение Вес Последовательность ACGCAAACGTTTtCtT -56 4, H. ducreyi cvpA-purF AaGCAAACGTTTGCGT -180 5, purHD cCGCAAcCGTTTGCtT -73 4, purM AaGCAAtCGgTTGCca -42 4, fhs AaGCAAACGTTTGCtT -89 4, deoC ACGCAAACGaTTaCGa -135 4, yhhQ ACGCAAACcTTTGCtT -85 4, HD ACGaAAACGaTTGCGa -294 4, V. cholerae prsA AaGCAAACGTTTGCtT -9 4, purC AaGCAAACGTTTtCtT -79 4, purT ACGCAAACGgTTGCtT -171 4, purL AgtaAAACGaTTGCGT 52 4, purB AaGCAAACGTTTGCtT -88 4, purEK AaGaAAACGTTTGCGT -65 4, cvpA-purF ACGCAAACGTTTtCcT -80 4, purMN tgcCAAACGaTTGCGc -186 3, purHD AgtCAAcCGaTTGCcT -113 3, guaBA gtGCAAACGTTTGCtT -94 4, gsk ggGCAAtCGaTTGttc -316 3, guaC AgtCAAACGTTTGCcT -261 4, ushA AgGaAAACGTTTGCGT -102 4, upp-uraA AtGtAAtCGcTTGCGc -155 3, pyrBI AaGCAAACGTTTGCcT -11 4, folD ACGaAAACGTTTGCGT -15 5, glyA AgGaAAACGTTTGCtT -98 4, serA gCGCAAACGTTTtCcT 3 4, fhs AaGCAAACGTTTGCtT -274 4, yicE gCGCAAACGTTTaCcT -217 4, ACGCAAcCGTTTaCtT -38 4, yieG AgGCAAtCGgTTGacT -104 3, xseA AgtgAAtCGTTTtCGT -151 3, cytR gCGCAAAgGTTTGCGc -193 3, pckA AgGCAAACGTTTaCtT -74 4, glpX AgGCAAACGTTTaCtT -74 4, ppsA tgGCAAtCGTTTGCtT -139 4, ydiA taGCAAACGTTTtCtT -71 4, VC ACGCcAtCGTTTGCtc -160 3, yhhQ cattAAACGTTTGCGT -289 3, HD ACGtAAACGaTTGCGa -243 4, V. fischeri prsA taGCAAtCGTTTGCtT -91 4, purC taGCAAACGTTTGCGT -73 4, purT ACGCAAACGgTTGCtT -183 4, purL ACGCAAACGaTTGCGg 146 4, purB ACGCAAACGTTTtCcT -87 4, purEK AaGaAAACGTTTGCGT -62 4, cvpA-purF ACGCAAACGTTTcCcT -81 4, purMN AtGCAAtCGaTTGCGc -207 4, purHD AgcCAAgCGTTTGCGc -121 3, guaBA Сайт Геном Оперон Положение Вес Последовательность gaGCAAACGTTTGCta -106 4, V. fischeri gsk taGCAAACGTTTaCtT -342 4, guaC ACGtAAACGgTTGCGT -221 4, ushA AgGgAAACGTTTGCGT -142 4, upp-uraA AaGCAAACGaTTaacT -62 3, pyrC AgcCAAACGTTTGCtc -246 4, pyrD AtGCAAACGcTTGCtT -227 4, pyrBI ACGCAAACGTTTGCtc -87 4, folD ACGtAAACGTTTGCGT -65 4, glyA cCGaAAcCGTTTGCtT -133 4, rpiA-serA AtGaAAACGaTTGCGT 44 4, fhs AaGCAAACGaTTGCtT -187 4, yicE ACGCAAcCGTTTaCtT -38 4, yieG ACGgtAACGaTTGCtT -104 3, yjcD AaaaAAcCGTTTtCGT 33 3, rnt gCGCAAACGcTTGgcT -103 3, xseA gaGCAAtCGTTTaCtT -47 4, cytR gCGCAAtCGaTTttaT -193 3, pckA AaGCAAACGTTTcCcT -75 4, glpX AgGgAAACGTTTGCtT -308 4, yiiU gCGtAAACGaTTGCGa -290 3, V. parahaemolyticus prsA AgGCAAACGTTTGCtT -133 4, purC AaGCAAACGTTTGCtT -82 4, purT ACGCAAACGgTTGCtT -160 4, purL gCGCAAACGTTTGCGc -88 4, purB ACGCAAACGTTTGCtT -89 4, purEK AaGaAAACGTTTGCGT -62 4, cvpA-purF ACGCAAACGTTTGCta -83 4, purMN tgcCAAACGaTTGCGc -197 3, purHD AgtCAAgCGaTTGCGT -138 3, guaBA caGCAAACGTTTGCta -95 4, gsk ACGCAAAgGTTTGCGc -299 4, guaC taGCAAACGTTTGCGT -113 4, upp-uraA gCGCAAgCGTTTGCtc -76 3, pyrC ctGaAAtCGaTTGCtT -193 3, pyrD AtGtAAtCGcTTGCGc -252 3, pyrBI ACGCAgACGTTgtgGT 95 3, speAB AgGCAAACGTTTGCGT -86 5, folD ACGaAAACGTTTGCGT -75 5, glyA AaGgAAACGTTTGtcc -107 3, serA gCGCAAACGTTTGCGc -84 4, fhs ACGCAAtCGgaTGCGT -296 4, gltS taGCAAACGaTTGCtT -165 4, yicE ACGCAAACGTTTagaT -45 4, yieG ACGgtAACGaTTGCtT -104 3, yjcD taGCAAACGTTTaacT -334 3, cytR gCGCAAAgGaTTGCGc -190 3, pck Сайт Геном Оперон Положение Вес Последовательность AgGCAAACGTTTaacT -74 4, V. parahaemolyticus glpX ACGtAAtCGTTTGCcT -76,00 4, pps AgGCAAACGaTTaCGT -138 4, ydiA taGCAAACGTTTGCtT -194,00 4, VC AaGaAcACtTTTtCGT -62,00 3, AgttAAACGTTTGCcT -287 3, yiiU ggatAAACGTTTGCGT -189 3, yhhQ V. vulnificus ACGgAAACGaTTGCGa -302 4, prsA AgGCAAACGTTTGCtT -135 4, purC AaGCAAACGTTTGCtT -82 4, purT ACGCAAACGgTTGCtT -158 4, purL gCGCAAACGTTTGCGc -90 4, purB AaGCAAACGTTTGCtT -88 4, purEK AaGaAAACGTTTGCGT -64 4, cvpA-purF gCGCAAACGTTTGCtc -80 4, purMN tgcCAAACGaTTGCGc -200 3, purHD gCGCAAgCGTTTGCGa -288 3, guaC taGCAAACGTTTGCta -92 4, gsk AgtCAAACGTTTGCcT -243 4, ushA gaGCAAACGTTTGCGc -108 4, upp taGCAAtCGTTTGCaa -66 3, uraA ACGgAAgCGTTTGCcT -49 3, pyrC AaGCAAACGTTTGCGT -85 5, folD ACGaAAACGTTTGCGT -72 5, glyA AaGgAAACGTTTGtcc -104 3, serA taGCAAACGaTTGCtT -185 4, yicE ACGCAAcCGTTTaCtT -39 4, yieG taGCAAACGTTTaCca -230 3, cytR gCGCAAAgGTTTGCGT -189 4, pck gCGtAAtCGTTTGCcT -33 4, pps AgGCAAACGaTTaCGc -138 4, ydiA taGCAAtCGTTTGCta -151 3, VC taGCAAACGTTTaCca -230 3, yiiU gttCAAtCGTTTGCGT -186 3, yhhQ ACGaAAACGaTTGCGa -287 4, P. profundum prsA AgGCAAACGTTTGCtT -142 4, purC AgGCAAACGTTTGCGT -72 5, purT gCGCAAACGgTTGCGT -199 4, purL taGCAAACGaTTGCGc -192 4, purB ACGCAAtCGaTTaCGT 61 4, purE AgGtAAACGTTTGCGT -62 4, cvpA-purF ACGCAAACGTTTcCcT -80 4, purMN gaGCAAACGTTTGCGT -169 4, purHD AgcCAAACGaTTGCtT -83 4, guaBA taGCAAAgGTTTGCGT -125 3, gsk ACGaAAACGaTTaCcT -64 4, guaC AgGgAAACGTTTGCGT -125 4, upp AgGtAAtCGaTTGCtT -65 4, uraA Сайт Геном Оперон Положение Вес Последовательность AgcCAAACGaTTGCGT -113 4, P. profundum pyrC ACGgAAtCGTTTGCGT -185 4, pyrD AaGCAAACGTTTGCGc -87 4, folD ACGaAAACGTTTGCGT -95 5, glyA gCGCAAACGaTTGCGT -97 4, fhs gCGCAAACGaTTGCtT -68 4, yicE taGCAAACGaTTaCGc -94 4, yjcD ACGCAAACGTTTaCtc -82 4, AaGCAAtCGTTTGgcT -191 4, xseA ACGCAAACGTTTcacT -22 4, glpX AtGCAAtCGTTTcCGT -66 4, pps ACGgAAACGaTTGCaT -134 4, ydiA AgGCAAACGaTTGCtT -155 4, yhhQ Приложение 9.


Множественное выравнивание для белков Fnr. Участки,соответствующие последовательности HTH, показаны белым шрифтом на темном фоне. Критически важные цистеиновые остатки показаны на голубом фоне. Условные обозначения: “*” – абсолютно консервативная позиция;

“:” – позиция с высокой степенью консервативности;

“.” – позиция с низкой степенью консервативности. Обозначения геномов: см. Табл. 2.

Fnr(ECO) -------------------MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDNIIERKKPIQKGQTLF Fnr(STY) -----MLKLTNINYGLSRPMIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDNIIERKKPIQKGQTLF Fnr(PCA) -------------------MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDNIIERKKPIQKGQALF Fnr(YPK) -------------------MIPEKRVIRRIQSGGYAIHCQDCSISQLCIPFTLNEHELDQLDNIIERKKPIQKGQALF Fnr(YEN) -------------------MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNENELDQLDNIIERKKPIQKGQALF Fnr(VPA) -------------------MISEKPATKRIQSGGCAIHCQDCSISQLCIPFTLNESELDQLDQIIERKKPIQKGQELF Fnr(VVU) -------------------MISEKPATKRIQSGGCAIHCQDCSISQLCIPFTLNEAELDQLDQIIERKKPIQKGQELF Fnr(VCH) -------------------MISEKPAAKRIQSGGCAIHCQDCSISQLCIPFTLNESELDQLDQIIERKKPIQKGQELF Fnr(VFI) -------------------MMSDNSANKRIQSGGCAIHCQDCSISQLCIPFTLNDSELDQLDEIIERKKPY-KGQELF Fnr(PMU) MILFEIDQFYIIFVVLIKVLFMDTKIGRKVQSGGCAIHCQDCSISQLCIPFTLNEQELDQLDNIIERKKPIQKSQVLF Fnr(AAC) ----------------MKILATETKLGRRVQSGGCAIHCQNCSISQLCIPFTLNQHELDQLDNIIERKKPIQKSQVLF Fnr(HIN) ----------------MKNFVAGAQSGRKIQSGGCAIHCQDCSISQLCIPFTLNEQELDQLDNIIERKKPIQKSQVLF Fnr(HDU) --------------------MKIISDAKNVGRTACTIHCQNCSISQLCLPFTLNEHELTQLDNIIERKKPVQKSQIIF :.:. :****:*******:*****: ** ***:******* *.* :* Fnr(ECO) KAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFDAIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMP Fnr(STY) KAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFDAIGSGHHPSFAQALETSMVCKIPFETLDDLSGKMP Fnr(PCA) KAGDELRSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFDAIGTAQHPSFAQALETSMVCEIPFETLDDLSGKMP Fnr(YPK) KAGDELKSLYAIRSGTIKSYTITEEGDEQITGFHLAGDLVGFDAISNLQHPSFAQALETSMVCEIPFDTLDDLSGKMP Fnr(YEN) KAGDELKSLYAIRSGTIKSYTITEEGDEQITGFHLAGDLVGFDAISNLQHPSFAQALETSMVCEIPFDTLDDLSGKMP Fnr(VPA) KAGDELKSLYAIRSGTIKSYTITEQGDEQITAFHLAGDLVGFDAITGDCHPSFAQALETSMVCEIPYEILDDLSGKMP Fnr(VVU) KAGDDLKSLYAIRSGTIKSYTITEQGDEQITAFHLAGDLVGFDAITGDSHPSFAQALETSMVCEIPSEILDDLSGKMH Fnr(VCH) KAGDELRSLYAIRSGTIKSYTITEQGDEQITAFHLAGDLVGFDAITGDQHPSFAQALETSMVCEIPYEILDDLSGKMP Fnr(VFI) KAGDELKCLYAIRSGTIKSYTITEQGDEQITAFHLAGDLVGFDAITEAQHPSFAQALETSMVCEIPYEILDDLSGKMP Fnr(PMU) KAGDPLNSLYAIRSGTIKTYTISETGEEQITSFQLPGDLVGFDAIMNMQHPSFAQALETSMVCEIPFDILDDLSGKMP Fnr(AAC) KAGDELTSLYAIRSGTIKSYTISETGEEQITSFHLPGDLVGFDAIMNMQHPSFAQALETAMVCEIPFDILDDLSGKMP Fnr(HIN) KAGDSLNSIYAIRSGTIKSYTISESGEEQITSFHLPGDLVGFDAITQMQHPSFAQALETAMVCEIPFDILDDLAGKMP Fnr(HDU) QSGDELRSIYAIRSGTIKSYTISESGEEQITAFHLPGNLVGFDAITNMRHLGFAQALETSMICEIPFDILDDLAGKMP ::** *.:*********:***:* *:****.*:*.*:******* *.*******:*:*:** : ****:***:

Fnr(ECO) NLRQQMMRLMSGEIKGDQDMILLLSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLL Fnr(STY) NLRQQMMRLMSGEIKGDQDMILLLSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLL Fnr(PCA) NLRQQMMRLMSGEIRGDQDMILLLSKKNAEERLAAFVYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLL Fnr(YPK) NLRQQIMRLMSGEIKGDQDMILLLSKKNAEERLAAFIYNLSHRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLL Fnr(YEN) NLRQQMMRLMSGEIKGDQDMILLLSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLL Fnr(VPA) KLRQQIMRLMSNEIKGDQEMILLLSKKNAEERLAAFLYNLSTRFSQRGFSPREFRLTMTRGDIGNYLGLTVETISRLL Fnr(VVU) KLRQQIMRLMSNEIKGDQEMILLLSKKNAEERLAAFLYNLSTRFSQRGFSPREFRLTMTRGDIGNYLGLTVETISRLL Fnr(VCH) KLRQQIMRLMSNEIKGDQEMILLLSKKNAEERLAAFLYNLSTRFSQRGFSPREFRLTMTRGDIGNYLGLTVETISRLL Fnr(VFI) KLRQQIMRLMSNEIKGDQEMILLLSKKNAEERLAAFLYNLSTRFHQRGFSPREFRLTMTRGDIGNYLGLTVETISRLL Fnr(PMU) KLRQQIMRLMSNEIKNDQEMILLLSKMNAEERLAAFIYNLSRRYSARGFSAREFRLTMTRGDIGNYLGLTVETISRLL Fnr(AAC) KLRQQIMRLMSNEIKSDQEMILLLSKMNAEERLAAFIYNLSQRYSARGFSAREFRLTMTRGDIGNYLGLTVETISRLL Fnr(HIN) KLRQQILRLMSSEIKSDQEMILLLSKMNAEERLAAFIHNLSKRYSARGFSAREFRLTMTRGDIGNYLGLTVETISRLL Fnr(HDU) KIRHQIMRLMSNEIKSDQEMILLLSKMNAEEKLAAFLHNLSQRYAERGFSAREFRLTMTRGDIGNYLGLTIETISRLL :*:*::****.**:.**:***** ** ****:****::*** *: ****.*******************:******* Fnr(ECO) GRFQKSGMLAVKGKYITIENNDALAQLAGHTRNVA----- Fnr(STY) GRFQKSGMLAVKGKYITIENSDALAALAGHTRNVA----- Fnr(PCA) GRFQKSGTLAVKGKYITIENIDALSELAGSSRK------- Fnr(YPK) GRFQKSNILSVKGKYITIENNEALAQLAGNPKPRL----- Fnr(YEN) GRFQKSDILSVKGKYITIENTEALAQLAGNPKPSCN---- Fnr(VPA) GRFQKSEILSVKGKYITILDHDALMELAGVTKE------- Fnr(VVU) GRFQKSEILSVKGKYIPILDHDALMELAGVSKE------- Fnr(VCH) GRFQKSEILSVKGKYITILNHAELMELAGVSKESK----- Fnr(VFI) GRFQKTEMLTVKGKYITINDHDALAELAGSAKEIK----- Fnr(PMU) GRLQKMGILSVQGKYITINDMVALIELSGTTKTNIKMGI- Fnr(AAC) GRFQKLGVLSVQGKYITINNMAELIELSGTNKNKIQLI-- Fnr(HIN) GRFQKLGVISVQGKYITINDLNGLIELTGTNKTKITLVK- Fnr(HDU) GRFQKSGMITVQGKYITINHMDKLTDMAGVTRSQISVTQSA **:** ::*:****.*. * ::* :

Приложение 10.

Множественное выравнивание для белков ArcA. Участки,соответствующие последовательности HTH, показаны белым шрифтом на темном фоне. Условные обозначения: “*” – абсолютно консервативная позиция;

“:” – позиция с высокой степенью консервативности;

“.” – позиция с низкой степенью консервативности. Обозначения геномов: см. Табл. 2.

ArcA(ECO) MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGKNGLLLARELREQANVAL ArcA(STY) MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGKNGLLLARELREQANVAL ArcA(YPK) MQTPHILIVEDEIVTRNTLKSIFEAEGYVVYEANDGAEMHHILSENDINLVIMDINLPGKNGLLLARELREQASVAL ArcA(YEN) MQTPHILIVEDEIVTRNTLKSIFEAEGYVVHEANDGAEMHHILSENDINLVIMDINLPGKNGLLLARELREQASVAL ArcA(PCA) MQTPHILIVEDELVTRNTLKSIFEAEGYVVHEATDGAEMHHILSENDINLVIMDINLPGKNGLLLARELREQATVAL ArcA(AAC) MQTPRILIVEDETITRNTLKSIFEAEGYEVFEASDGTQMHQVLDTEDIHLVIMDINLPGKNGLMLARELRENADIAL ArcA(PMU) MGTPQILIVEDEAITRNTLKSIFEAEGYEVFEAADGAQMHRILSNKVINLVIMDINLPGKNGLMLARELRETTNTAL ArcA(HIN) MTTPKILVVEDEIVTRNTLKGIFEAEGYDVFEAENGVEMHHILANHNINLVVMDINLPGKNGLLLARELREELSLPL ArcA(HDU) MQNPQILIVEDELVTRNTLKSIFEAEGYEVFEASDGTEMNTILAQQTINLVIMDINLPGKNGLMLARELRENTKMAL ArcA(VPA) MQTPQILIVEDEQVTRNTLKSIFEAEGYAVFEASDGEEMHQVLSDNSINLVIMDINLPGKNGLLLARELREQANIAL ArcA(VCH) MQTPQILIVEDEQVTRNTLKSIFEAEGYAVFEASNGEEMHQVLSDYPINLVIMDINLPGKNGLLLARELREQADVAL ArcA(VVU) MQTPQILIVEDEQVTRNTLKSIFEAEGYAVFEASNGDEMHHMLSENSINLVIMDINLPGKNGLLLARELREQADVAL ArcA(VFI) MQTPQILIVEDEHVTRNTLKSIFEAEGYNVFEASDGEEMHKVLSEQQLNLVIMDINLPGKNGLLLARELRERGDMAL *.*:**:**** :******.******* *.** :* :*: :* ::**:***********:*******.* ArcA(ECO) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGP ArcA(STY) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGP ArcA(YPK) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRTMNLSSVGEERRLVESYKFNGWELDINSRSLVSP ArcA(YEN) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRTMNLGSVGEERRLVESYKFNGWELDINSRSLISP ArcA(PCA) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRTMNLGSGTEERRLVESYRFNGWELDINSRSLISP ArcA(AAC) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLQRTMQENQR-THH--VEQYKFNGWTLDLNSRTLVTP ArcA(PMU) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLQRTMQENSKDSHHP-IEQYRFNGWTLDLNSRTLINP ArcA(HIN) IFLTGRDNEVDKILGLEIGADDYLTKPFNPRELTIRARNLLHRAMP-HQEKENTFGREFYRFNGWKLDLNSHSLITP ArcA(HDU) MFLTGRDNEVDKILGLEVGADDYITKPFNPRELTIRARNLLHRTMLEKDKVSHSEQQESYRFNGWTLDVNSRSLITP ArcA(VPA) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRSMSTNAVQEEKRSVEKYEFNGWVLDINSRSLVSP ArcA(VCH) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRSMHAGTTQEEKRSVEKYVFNGWELDINSRSLVSP ArcA(VVU) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRSMSSSVMPEEKRSVEKYEFNGWVLDINSRSLISP ArcA(VFI) MFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLTRSMNQNTVVEDKKMVERYVFNGWTVEINSRSLVSP :****************:*****:***************** *:*. * * **** :::**::*: * ArcA(ECO) DGEQYKLPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYR ArcA(STY) DGEQYKLPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYR ArcA(YPK) TGEHYKLPRSEFRAMLHFCENPGKIQSRGELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYR ArcA(YEN) AGEHYKLPRSEFRAMLHFCENPGKIQSRGELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYR ArcA(PCA) AGEQYKLPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTADTPEIIATIHGEGYR ArcA(AAC) EGEERKLPRSEFRAMLHFCENPGKIQTREELLKKMTGRELKPQDRTVDVTIRRIRKHFEDHHETPEIIATIHGEGYR ArcA(PMU) EGEEYKLPRSEFRAMLHFCENPGKIQTREELLKKMTGRELKPQDRTVDVTIRRIRKHFEDHPETPEIIATIHGEGYR ArcA(HIN) EGQEFKLPRSEFRAMLHFCENPGKLQTREELLKKMTGRELKPQDRTVDVTIRRIRKHFEDHPNTPNIIMTIHGEGYR ArcA(HDU) EGEINKLPRSEFRALLHFCENPGKIQTREDLLLKMTGRELKPHDRTVDVTIRRIRKHFEDHLNTPEIIVTIHGEGYR ArcA(VPA) AGDSYKLPRSEFRALLHFCENPGKIQTRADLLKKMTGGELKPHDRTVDVTIRRIRKHFESVSGTPEIIATIHGEGYR ArcA(VCH) DGDSYKLPRSEFRALLHFCENPGKIQTRADLLKKMTGRELKPHDRTVDVTIRRIRKHFESVSGTPEIIATIHGEGYR ArcA(VVU) SGDGYKLPRSEFRALLHFCENPGKIQTRADLLKKMTGRELKPHDRTVDVTIRRIRKHFESVSGTPEIIATIHGEGYR ArcA(VFI) SGESYKLPRSEFRALLHFCENPGKIQTRADLLKKMTGRELKPHDRTVDVTIRRIRKHFESVTDTPEIIATIHGEGYR *: *********:*********:*:* :** *********:****************. **:** ******** ArcA(ECO) FCGDLED ArcA(STY) FCGDLQD ArcA(YPK) FCGDLEE ArcA(YEN) FCGDLEE ArcA(PCA) FCGDLEN ArcA(AAC) FCGDLES ArcA(PMU) FCGELE- ArcA(HIN) FCGDIE- ArcA(HDU) FCGQIE— ArcA(VPA) FCGDLED ArcA(VCH) FCGDLED ArcA(VVU) FCGDLEA ArcA(VFI) FCGDLEEL ***:::

Приложение 11.

Множественное выравнивание для белков ArcB. Участки,соответствующие последовательности HTH, показаны белым шрифтом на темном фоне. Критически важные цистеиновые остатки показаны на голубом фоне. Условные обозначения: “*” – абсолютно консервативная позиция;

“:” – позиция с высокой степенью консервативности;

“.” – позиция с низкой степенью консервативности. Обозначения геномов: см. Табл. 2.

ArcB(ECO) MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTMVLHGQVESIDVIRSIFFGLLITPWAVYFLSVVV ArcB(STM) MKQIRMLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTMVLHGQVESIDVIRSIFFGLLITPWAVYFLSVVV ArcB(STY) MKQIRMLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTMVLHGQVESIDVIRSIFFGLLITPWAVYFLSVVV ArcB(YPK) MKQIRVLAQYYVDLMVKLGLVRFSLLLASALVLLAMVVQMAVTFVLRGSVETLDLVRSIFFGLLITPWAVYFLSVVV ArcB(YEN) MKQIRVLAQYYVDLMVKLGLVRFSLLLASVLVLLAMVVQMAVTFVLSGSVETLDLVRSIFFGLLITPWAVYFLSVVV ArcB(PCA) MKQIRLLAQYYVDLMVKLGLVRFSLLLASVLVLLAMVVQMAVTLLLSGEVENIDVVRSIFFGLLITPWAVYFLSVVV ArcB(VPA) MKPMKNLAQYYVDLLVKLGIVRFSILLALALVALAVVVQVGITLALKGRVDDIDIVRSVFFGLLITPWAVYFLSVVV ArcB(VVU) MKPMKNLAQYYVDLLVKLGIVRFSMLLALALVALAVVVQVGITLVLKGNVDDIDIVRSVFFGLLITPWAVYFLSVVV ArcB(VCH) MKPMKNLAQYYVDLLVKLGILRFSILLALALVALAVVVQVGITLILNGFVDDIDIIRSVFFGLLITPWAVYFLSVVV ArcB(VFI) MKQLKILAQFYVDLMVRLGIVRFSLLLAIALVALAVIVQVGVTLVLSGEVNDLDIIRSVFFGLLITPWAVFFLSVVV ArcB(AAC) MKNLKDFVRRYVDWVIRLGRIRFSLLGVMVLAVLALCTQILLSLLVMGKILWADVARSIVFGLISAPFVIYFFTVLV ArcB(PMU) MKNIKYFAQKYVDWVIRLGRLRFSILGVIVLAILALCTQALLSLFIIGEVYWVDMIRSVVFGLISAPFVIYFFTVLV ** :: :.: *** :::** :***:*..*. **:.* ::: : * : *: **:.***: :*:.::*::*:* ArcB(ECO) EQLEESRQRLSRLVQKLEEMRERDLSLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQS ArcB(STM) EQLEESRQRLSRLVQKLEEMRERDLKLNVQLKDNIAQLNQEIADREKAEAELQETFEQLKVEIKEREEAQIQLEQQS ArcB(STY) EQLEESRQRLSRLVQKLEEMRERDLKLNVQLKDNIAQLNQEIADREKAEAELQETFEQLKVEIKEREEAQIQLEQQS ArcB(YPK) EQLEESRQRLSRLVDKLEVMRHRDLELNKQLTENITQLNQEIVEREKAEKAHLQVVDKLKEEMGHREQAQIELGQQS ArcB(YEN) EQLEESRQRLSRLVDKLEVMRYRDLELNKQLTENIAQLNQEIVEREKAEKAHLQVVDKLKEEMGHREQAQVELGQQS ArcB(PCA) EQLEESRQRLSKLVAKLEEMRHRDLELNVQLQENIAQLNQEIADRIKAEEARVLVMSRLKEEMSRREQAQIELEQQS ArcB(VPA) DQLEESRQRLTKLVSKLKDMRSRDQELNFQLQQNIEKLNLEIEERIKAEEAREEAMQDLENEVFQRERTQLELAERT ArcB(VVU) DQLEESRQRLSKLVSKLKDMRSRDQELNVQLQQNIEQLNQEIEERIRAEEAREEAMKDLENEVFQRERTQVELAERT ArcB(VCH) DQLEESRQRLSKLVSKLKDMRSRDQELNQKLQQNIVKLNQEIEERIKAEEAREEAMADLENEVYQREKTQVELAERT ArcB(VFI) EHLEDSRQRLSKLVSKLEEMRTRDLDLNKKLKGNVKKLNQEIEEREKAEEARQEAMEDLENEVFQREKAQLELAEQT ArcB(AAC) EKLEHSRQALS---------------------CSVEELCQERVSAEKKLSEALDN------------------ ArcB(PMU) EKLERSRLGLA---------------------KMVENLRKEVSERVLAEKKLSIALDN------------------ ::** ** *: : :* *: * **..

ArcB(ECO) SFLRSFLDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEKVFRHNVSLTYEQW ArcB(STM) SFLRSFLDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPEDVYSPEAAEKVIETDEKVFRHNVSLTYEQW ArcB(STY) SFLRSFLDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPEDVYSPEAAEKVIETDEKVFRHNVSLTYEQW ArcB(YPK) ALLRSFLDASPDLVYYRNEDNEFSGCNRAMELLTGKSEKQLVGLTPKDVYAPDIAEKVMETDEKVFRHNVSLTYEQW ArcB(YEN) ALLRSFLDASPDLVYYRNEDNEFSGCNRAMELLTGKSEKQLVGLTPKDVYAPDIAEKVMETDEKVFRHNVSLTYEQW ArcB(PCA) ALLRSFLDASPDLVYYRNEEKEFSGCNRAMELLVGKSQKQLIGLTPQDVYAPDIAEKVMETDEKVFRHNVSLTYEQW ArcB(VPA) ALLRSFIDASPDLIYYRNADGVFSGCNRAMEELTGKKEHQLVGLSPWDVYSKEIAQPIVDTDQKVFSDNQAITYEQW ArcB(VVU) ALLRSFIDASPDLIYYRNAEGVFSGCNRAMEELTGKKEHQLVGLSPWDVYSKEVAQQIVDTDQQVFANNRALTYEQW ArcB(VCH) ALLRSFIDASPDLIYYRNAKGEFSGCNRAMEELTGKRESELVGLTPWDVYRKEIAQSIVETDQQVFNNNTAITYEQW ArcB(VFI) ALLRSYLDTSPDIIYYRNDLGLFSGCNKAMEQLTGKLEKELIGLSPKDVYDAEVAAKVIDTDRQVLQENQALTYEQW ArcB(AAC) ---------------------------------------------------------------------------- ArcB(PMU) ---------------------------------------------------------------------------- ArcB(ECO) LDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDALERASRDKTTFISTISHELRTPLNGIVGLSRIL ArcB(STM) LDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDALERASRDKTTFISTISHELRTPLNGIVGLSRIL ArcB(STY) LDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDALERASRDKTTFISTISHELRTPLNGIVGLSRIL ArcB(YPK) LVYPDGRKACFELRKVPFYDRVGKRHGLMGFGRDITERKRYQDALENASRDKTTFISTISHELRTPLNGIVGLSRIL ArcB(YEN) LVYPDGRKACFELRKVPFYDRVGKRHGLMGFGRDITERKRYQDALENASRDKTTFISTISHELRTPLNGIVGLSRIL ArcB(PCA) LVYPDGRKACFELRKVPFYDRMGKRHGLMGFGRDITERKRYQDALENASRDKTTFISTISHELRTPLNGIVGLSRIL ArcB(VPA) LEYPDGRKHYFELRKVPFYSKDGRHLGLVGFGRDITERKRHEESLEKASRDKTTFISTISHELRTPLNGIVGLSRIL ArcB(VVU) LEYPDGRKHYFELRKVPFYSKDGRHLGLVGFGRDITERKRHEESLEKASRDKTTFISTISHELRTPLNGIVGLSRML ArcB(VCH) LEYPDGRKSFFELRKVPFYNKDGRHLGLVGFGRDITERKRHEESLEKASRDKTTFISTISHELRTPLNGIVGLSRML ArcB(VFI) LDYPDGRKACFELNKIPFYSKDGRRLGLVGFGRNITERKEYEDALQKASRDKTTFISTISHELRTPLNGIVGLSRML ArcB(AAC) --------------------------------------------LEKINRDKTTLMTTISHELRTPLNGIIGLSRIL ArcB(PMU) --------------------------------------------IEKTSRDKTRLMATISHELRTPLNGIIGLSRIL ::..**** :::*************:****:* ArcB(ECO) LDTELTAEQEKYLKTIHVSAVTLGNIFNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPT ArcB(STM) LDTDLTAEQEKYLKTIHVSAVTLGNIFNDIIDMDKMERRKVQLDNQPIDFTSFMADLENLSGLQAQQKGLRFNLEPT ArcB(STY) LDTDLTAEQEKYLKTIHVSAVTLGNIFNDIIDMDKMERRKVQLDNQPVDFTSFMADLENLSGLQAQQKGLRFNLEPT ArcB(YPK) LDTELDAEQLKYLKTIHVSAITLGNIFNDIIEMDKLERRKVQLDNQPVDFTGFMADLENLSGLLVQPKGLKFIMEPQ ArcB(YEN) LDTELDSEQLKYLKTIHVSAITLGNIFNDIIEMDKLERRKVQLDNQPIDFTGFMADLENLSGLLVQPKGLKFIMEPQ ArcB(PCA) LDTQLDLEQQKYLKTIHVSAITLGNIFNDVIEMDKQERRKVQLDNQPIDFTGFLVDLENLGGLLAEQKGLKLIMDQH ArcB(VPA) LDSQLTEEQRKHMQTINVSAITLGNIFNDIIDMDKFDRRKLELLPAPLNFEDFVAEIESISALMAEQKGLRFDLERL ArcB(VVU) LDTRLTDEQRKYMQTINVSAITLGNIFNDIIDMDKFDRRKLELLPAPLNFEDFVAEIESISALMAEQKGLRFDLERL ArcB(VCH) LDTPLSGEQRKHLQTINVSAVTLGNIFNDIIDMDKFDRRKLELFPAALNFEEFVAEIEVLAALMAEQKGLRFDLERL ArcB(VFI) LDSRLDDEQHNYMRTINVSAITLGNIFNDIIDMDKFDRRKLELFPKPLDFISFVTEMENISGLMAEQKGLRFDLERL ArcB(AAC) LEENPSERQQNYLKTINSSTLSLAHIFSDIIDLEKIDAKRIELNRKATDLYALLNDIANFALLMTEEKHLQFQLVCP ArcB(PMU) LDEKLTETQQNYLKTIHVSAISLGHIFSDIIDLEKIDARRIELNAKAVELNSFLNDISNVARLLAQQKKLAFQLVTD *: * ::::**: *:::*.:**.*:*:::* : ::::*. :: :: ::.. *.: * * : :

ArcB(ECO) LPLPHQVITDGTRLRQILWNLISNAVKFT------QQGQVTVRVRYDEGD---MLHFEVEDSGIGIPQDELDKIFAM ArcB(STM) LPLPHKVITDGTRLRQILWNLISNAVKFT------QQGQVTVRARYDEGD---MLHFEVEDSGIGIPQDEQDKIFAM ArcB(STY) LPLPHKVITDGTRLRQILWNLISNAVKFT------QQGQVTVRARYDEGD---MLHFEVEDSGIGIPQDEQDKIFAM ArcB(YPK) LPLPEKVIADGTRLRQILWNLIGNAVKFT------QQGKIVVRVRREGND---RLIFEVEDSGMGIPEDEQDKIFAM ArcB(YEN) LPLPEKVITDGTRLRQILWNLIGNAVKFT------QQGEIVVRVRRESND---RLIFEVEDSGMGIPEDEQDKIFAM ArcB(PCA) QPLPQKVITDGTRLRQILWNLLSNAVKFTPKGEKGEKGEIVVRVWHEKGD---RLRFEVEDSGMGIPADELEKIFAM ArcB(VPA) SDLPKAIEVDATRLRQVIWNLVSNAMKFT------KEGGVVMTVSADVEEDFATIIMEVEDTGIGIPEAELEKIFAM ArcB(VVU) SELPKAIEVDATRLRQVIWNLISNAMKFT------KEGGVVMTVSSEVEEDFATIIIEVEDTGIGIPEAELEKIFAM ArcB(VCH) TDLPSLVEVDSTRLRQVLWNLLSNAMKFT------KEGGVVMTVSAECDAQHAEITIEVEDTGIGIPEAEQEKIFAM ArcB(VFI) TDLPPHISVDSTRLRQVLWNLVGNATKFT------KEGGVVMSVSADIHQDSAEITFEIEDTGIGIPEEELEKIFAM ArcB(AAC) PTLPNWLMLDGVRLNQVLWNLINNAVKFT------QQGSVTLSVEQTAEE---EFALRVTDTGIGIVEQDLQKIFEL ArcB(PMU) KNLPTWFMVDYARLSQILWNLIGNAVKFT------QEGRITLNVKRVSEY---QISFSVSDTGIGIPANELPRVFEM **. *.** *::***:.** *** ::* :.:. : : : *:*:** :.:* :



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